Medium composition

[Water]
APW medium
Medium composition; 0.5 mM NaCl, 0.2 mM NaHCO3, 0.05 mM KCl, 0.4 mM CaCl2
Purpose for use; artificial pond water

ASPM medium (Guillard, 1983)
Medium composition;
[7ppt salinity]: NaCl 5.28g, KCl 0.17g, CaCl2 0.25g, MgCl2/6H2O 0.92g, MgSO4/7H2O 1.12g, NaHCO3 0.08g, in 1 L of NANOpure deionized water, pH 7.5
[15ppt salinity]: NaCl 11.31g, KCl 0.36g, CaCl2 0.54g, MgCl2/6H2O 1.97g, MgSO4/7H2O 2.39g, NaHCO3 0.17g, in 1 L of NANOpure deionized water, pH 8.0
Purpose for use; Synthetic seawater

Chalkley's medium
Medium composition; 0.01% (w/v) sodium chloride, 0.0004% (w/v) potassium chloride, 0.0006% (w/v) calcium chloride
Purpose for use; isotonic inorganic salt solution

EPA medium (EPA, 1985)
Medium composition; NaHCO3 96 mg, CaSO4/2H2O (or CaSO4) 60 mg, MgSO4 60 mg, KCl 4 mg in deionized water 1L. Deionized base water was obtained from a Barnstead NANOpure II system with one pretreatment, one high-capacity, and two ultrapure cartridges. pH 7.5
Purpose for use; Synthetic freshwater


[Soil]
ASTM artificial soil (ASTM, 1997)
Soil composition; 10% sphagnum peat moss, 20% kaolin clay, 70% sand (grade 70. particle size 0.1-0.3 mm)
The ASTM soil is of the same composition as the OECD soil (OECD 1984) and serves as a standard test medium. (ref. ID; 3301)

OECD artificial soil (OECD Guideline No. 207) (OECD, 1984)
Soil composition;
1. 10% < 0.5 mm ground sphagnum peat, 20% kaolin clay, 69% fine sand, 1% calcium carbonate (by dry weight)
2. 70% sand, 20% kaolin clay, 10% organic matter (as Sphagnum peat) (by dry weight), pH 6.0 +/- 0.5 (prepared by powdered calcium carbonate)