Culture Collection History

The organisms listed here were collected mainly biological sewage treatment systems, activated sludge, biofilm and so on.
We don't supply these organisms.

Protozoa
Amoeba sp., Arcella sp., Aspidisca sp., Asterocaelum sp., Blephalism sp., Carchesium sp., Centropyxis sp., Colpidium sp., Colpoda sp., Dileptus sp., Epistylis sp., Euglypha sp., Euplotes sp., Glaucoma sp., Gonostomum sp., Hemiophrys sp., Opercularia sp., Oxytricha sp., Paramecium sp., Peranema sp., Pseudoblepharisma sp., Spirostomum sp., Stentor sp., Tetrahymena sp., Trithigmostoma sp., Vorticella sp., Hypotrichida

Rotifera
BDELLOIDEA (Adineta sp., Habrotrocha sp., Philodina sp., Rotaria sp.)
MONOGONONTA (Cephalodella sp., Colurella sp., Lecane sp., Lepadella sp.)

Oligochaeta
Aeolosoma sp., Dero sp., Nais sp., Pristina sp.


Protozoa

Amoeba sp.
Sampling site: Johkasou (Terunuma)
Isolated by: Kuniyasu, Yuko (1996.02)
Death: 2015?
Culture medium: 1% Agar layer + LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: This species is not observed pseudopodium in liquid medium only. Agar layer is necessary.

Arcella sp.
Sample: Biofilm of Gappei-shori johkasou experimental system in Bio-Eco.
Isolated by: Kuniyasu, Yuko (2010.09.10)
Death: 2015?
Culture medium: BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Cell expanding pseudopodium was rarely observed under the culture conditions.

Aspidisca costata (Dujardin) [As-1]
Sampling site: Ditch in NIES
Isolated by: Kuniyasu, Yuko (1986.04.09)
Death: 1989.06
Culture medium: LE medium
Food: Bacteria
Culture vessel: Tube and Petri dish (diameter 46 mm)
Temperature: 10 degrees C
Light condition: Dark

Asterocaelum sp. [Ana No.1]
Sampling site: The Yabakei Dam in Ooita Prefecture
Isolated by: Kuniyasu, Yuko (1989.12)
Culture medium: MA, M11-medium (for algae) and BLEY medium (for bacteria)
Food: Anabaena (NIES No.1, No.77) and bacteria
Culture Vessel: Petri dish
Temperature: 25 (for algae) and 20 (for bacteria) degrees C
Light condition: Approx. 1,000 lux, continuous (for algae) and dark (for bacteria)
Notes: The life cycle of Asterocaelum sp. is not completed under the culture conditions.


Blephalisma japonicum
Sampling site: Rice field
Isolated by: Miura, Yasuhiro (1996)
Identified by: Takahashi, Tadao
Death: 2017.04
Culture medium: LE medium + a bit of rice straw, BLEY medium
Food: Bacteria + Flagellata (F-1)
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Red coloured.

[Blepha-1]
Sample: Slude of OD experimental equipment in NIES laboratory (room temperature 20 degrees C)
Isolated by: Kuniyasu, Yuko (1986.07.04)
Death: 1994
Culture medium: 1/2LE medium + trace stigumasterol (50 ul stock solution/50 ml medium)
Food: Bacteria + Flagellata (F-1)
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10 degrees C
Light condition: Dark
Notes: Red coloured.


Carchesium sp. [Car-1]
Sampling site: Rural sewage treatment plant (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1986.12.05)
Death: 1987.06
Culture medium: 1/2LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Small colony (< 10 cells in a colony).

Centropyxis sp.
Sampling site: (Chikayama)
Isolated by: Kuniyasu, Yuko (1997.12.01)
Death: 2011.09.20
Culture medium: 1% Agar layer + CE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Cell expanding pseudopodium was rarely observed under the culture conditions.


Colpidium campylum [Colpi-1]
Sample: Activated slude of the Sunamachi Municipal Sewage Treatment Plant in Tokyo (Sudo, Ryuichi)
Isolated by: Sudo, Ryuichi (1970s)
Identified by: Sudo, Ryuichi
Culture medium: LE medium, BLEY medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10, 20 degrees C
Light condition: Dark

[Colpi-2]
Sampling site: Anaerobic tank of Gappei-shori johkasou (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1987.04)
Culture medium: LE medium, BLEY medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10, 20 degrees C
Light condition: Dark

[Colpi-3]
Sampling site: Pig farm wastewater treatment plant using Gappei-shori johkasou (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1992.06.26)
Culture medium: 1/2LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[Colpi-Nagasaka]
Sampling site: The hair dresser's parlor wastewater treatment plant using Gappei-shori johkasou in Hitachinaka City, Ibaraki Prefecture (Nagasaka)
Isolated by: Kuniyasu, Yuko (1997.02)
Culture medium: 1/2LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
References: Journal of Japanese Society of Water Treatment Biology Vol.43(4):181-188 (2007)


Colpoda sp. [Colpo-1]
Sample: Activated slude of the Sunamachi Municipal Sewage Treatment Plant in Tokyo (Sudo, Ryuichi)
Isolated by: Sudo, Ryuichi (1970s)
Culture medium: LE medium, BLEY medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10, 20 degrees C
Light condition: Dark
Notes: Cyst formation.

[Colpo-2]
Sample: Sludge of anaerobic filter experimental equipment in NIES laboratory (room temperature 20 degrees C)
Isolated by: Kuniyasu, Yuko (1987.03.16)
Death: 1994.04
Culture medium: LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Cyst formation. Not suitable 10 degrees C.

[Colpo-Kei]
Sampling site: Experimental equipments (No.4) for wastewater treatment system using common reed in NIES field (Kei)
Isolated by: Kuniyasu, Yuko (2005.06.22)
Culture medium: BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Cyst formation.

[Colpo]
Sampling site: Trenches for wastewater treatment? (Inamori, Yuhei)
Isolated by: Inamori, Yuhei
Culture medium: LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Cyst formation.


Dileptus anser [Dil-1]
Sample: Sludge of landfill discharge treatment system in Nagoya City, Aichi Prefecture (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1986.09.25)
Death: 1988.11
Culture medium: LE medium
Food: Bacteria + Tetra-1, Bacteria + Colpi-1, Bacteria + Aspi-1
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20, 30 degrees C
Light condition: Dark
Notes: Subculture frequency is once a week. Good growth occurs at high temperatures. Predatory-prey interaction.
References: 24th Japanese Society of Water Treatment Biology (1987)
Epistylis sp. [Epi-1]
Sampling site: Anoxic tank in Berlin (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1988.10.31)
Death: 1990.06
Culture medium: 1% Agar layer + LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: Good growth occurs at 20 degrees C. 10 degrees C is not suitable.
Light condition: Dark

[Epi-2]
Sampling site: Anaerobic tank in Berlin (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1988.10.31)
Death: 1990.06
Culture medium: 1% Agar layer + LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark


Euglypha sp.
Sampling site: Experimental equipments (Run 1) of Mr. Jyouno in Bio-Eco
Isolated by: Kuniyasu, Yuko (2010.09)
Death: 2016.03.25
Culture medium: 1/2LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10, 20 degrees C
Light condition: Dark
Notes: Cell expanding pseudopodium was rarely observed under the culture conditions.
Euplotes aediculatus [Euplo-1]
Sample: Sludge of OD experimental equipment in NIES laboratory (room temperature 20 degrees C)
Isolated by: Kuniyasu, Yuko (1986.03.28)
Death: 1994.04
Culture medium: LE medium
Food: Bacteria + Flagellata (F-1)
Culture vessel: Tube
Temperature: 10 degrees C
Light condition: Dark
Notes: Body length 110-120 um, body width 80 um.

[Euplo-2]
Sample: Sludge of the Kohoku Sewage Treatment Plant in Ibaraki Prefecture
Isolated by: Kuniyasu, Yuko (1986.05.09)
Death: 1994.04
Culture medium: LE medium
Food: Bacteria + Flagellata (F-1)
Culture vessel: Tube
Temperature: 10 degrees C
Light condition: Dark

[Euplo-3]
Sampling site: Aerobic tank of Gappei-shori johkasou (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1987.04)
Death: 1988
Culture medium: 1/2LE medium
Food: Bacteria + Flagellata (F-1)
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10 degrees C
Light condition: Dark

[Euplo-4]
Sampling site: Anaerobic contacted filter experimental equipment in NIES laboratory (room temperature 20 degrees C)
Isolated by: Kuniyasu, Yuko (1987.03.30)
Death: 1996.04.22
Culture medium: LE medium
Food: Bacteria + Flagellata (F-1)
Culture vessel: Tube
Temperature: 10 degrees C
Light condition: Dark

[Euplo-5]
Sample: Sludge of OD experimental equipment in NIES laboratory (room temperature 20 degrees C)
Isolated by: Kuniyasu, Yuko (1991.12.06)
Death: 1994.09
Culture medium: 1/2LE medium
Food: Bacteria + Flagellata (F-1)
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark


Glaucoma sp.?
Sample: Sludge of Joukasou (Chikayama)
Isolated by: Kuniyasu, Yuko (1997.02.21)
Culture medium: LE medium >> BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

Gonostomum sp. [Gono-1]
Sampling site: Pond in NIES
Isolated by: Kuniyasu, Yuko (1986.04.28)
Death: 1990.6
Culture medium: 1/2LE medium
Food: Bacteria + Flagellata (F-1)
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

Hemiophrys pleurosigma
Sample: Sludge of rural sewage treament plant (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1986.12.05)
Identified by: Ouchiyama, Takahiro
Death: 1987.06
Culture medium: 1/2LE medium
Food: Vorticella sp. [Vor-3] + Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Predatory-prey interaction. The prey growth is lower than the capture rate of H. pleurosigma in same culture vessel.

Opercularia sp. [Ope-1]
Sample: Activated sludge of the Morigasaki Municipal Sewage Treatment Plant in Tokyo (Sudo, Ryuichi)
Isolated by: Sudo, Ryuichi (1970s)
Culture medium: LE medium, BLEY medium
Food: Bacteria
Culture vessel: Tube or Petri dish (diameter 46 mm)
Temperature: 10, 20 degrees C
Light condition: Dark


Oxytricha fallax Stein [Oxy-1]
Sampling site: The contacted aeration experimental equipment in NIES laboratory (room temperature 20 degrees C)
Isolated by: Kuniyasu, Yuko (1986.06.27)
Death: 1994
Culture medium: LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Good growth occurs at 20 degrees C. 10 degrees is not suitable.

[Oxy-2]
Sample: Sludge of aeration tank of Gappei-shori johkasou (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1987.04.06)
Death: 1994.04
Culture medium: LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Good growth occurs at 20 degrees C. 10 degrees is not suitable.

[Oxy-3]
Sampling site: River in Oomuta City (Koga)
Isolated by: Kuniyasu, Yuko (1988.03.25)
Death: 1994.04
Culture medium: LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Good growth occurs at 20 degrees C. 10 degrees is not suitable.


Paramecium aurelia [Para-1]
Sample: Sludge of OD experimental equipment in NIES laboratory (room temperature 20 degrees C)
Isolated by: Kuniyasu, Yuko (1986.04.04)
Death: 1994.04
Culture medium: LE medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10 degrees C
Light condition: Dark

Paramecium bursalia (Focke) [Para-2]
Sampling site: Ditch in NIES
Isolated by: Kuniyasu, Yuko (1986.04.09)
Death: 1988.12
Culture medium: LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Approx. 1,500 lux, continuous

Paramecium caudatum (Ehrenberg)
Sampling site: Pond at side green-house in NIES (Kuniyasu, Yuko)
Isolated by: Kuniyasu, Yuko (1984.04.25)
Death: 1990?
Culture medium: LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[Para-3]
Sample: Sludge of anaerobic tank of Gappei-shori johkasou (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1987.03.31)
Death: 1994.09
Culture medium: LE medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10 degrees C
Light condition: Dark

[Para-4]
Sample: Sludge of the treatment plant of Nagaoka Sangyo (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1986.12.05)
Death: 1993.03
Culture medium: LE medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10 degrees C
Light condition: Dark

[Para-5]
Sample: Sludge of rural sewage treatment plant (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1986.12.05)
Death: 1996.09
Culture medium: LE medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10 degrees C
Light condition: Dark

[Para-6]
Sampling site: The anaerobic filter experimental equipment in NIES laboratory (room temeprature 20 degrees C)
Isolated by: Kuniyasu, Yuko (1987.03.30)
Death: 1988.08.24
Culture medium: LE medium
Food: Bacteria
Culture vessel: Tube or Petri dish (diameter 46 mm)
Temperature: 10 degrees C
Light condition: Dark

[Para-7] Paramecium polycarum Woodruff & Spencer
Sample: Activated sludge of The Mikawashima Municipal Sewage Treatment Plant in Tokyo (Sudo, Ryuichi)
Isolated by: Sudo, Ryuichi (1968.10)
Identified by: Sudo, Ryuichi (1968.10)
Death: 1989.10
Culture medium: LE medium
Food: Bacteria
Culture vessel: 50 ml flask or Tube
Temperature: 10, 20 degrees C
Light condition: Dark

[Para-8]
Sampling site: Pond in NIES
Isolated by: Kuniyasu, Yuko (1992)
Death: 1994.04
Culture medium: 1/2LE medium
Food: Bacteria + Algae
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 25 degrees C
Light condition: Approx. 1,000 lux, continuous

[Para] Paramecium sp.?
Sampling site: Johkasou (Chikayama)
Isolated by: Kuniyasu, Yuko (2003.3.14)
Culture medium: 1/2LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C


Peranema sp.
Sampling site: The hair dresser's parlor wastewater treatment plant using Gappei-shori johkasou in Ishioka City, Ibaraki Prefecture (Nagasaka)
Isolated by: Kuniyasu, Yuko (1997.02.05)
Death: 2008.05
Culture medium: 1/2LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

Pseudoblepharisma tenuis [Pblepha-1]
Sampling site: Aeration tank of Gappei-shori johkasou (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1987.04.09)
Death: 1997.03.10
Culture medium: 1/2LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10 degrees C
Light condition: Dark
Notes: Body is red-coloured.


Spirostomum teres Claparede & Lachmann [Spiro-1]
Sample: Sludge of the Gappei-shori johkasou in Yachiyo city, Chiba Prefecture (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1989.06.18)
Death: 1993
Culture medium: 1% Agar layer + 1/2LE medium
Food: Bacteria + Flagellata (F-1)
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[Spiro-2]
Sample: Sludge of the Gappei-shori johkasou in Yusato (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1991.05.22)
Death: 1992.01
Culture medium: 1% Agar layer + 1/2LE medium
Food: Bacteria + Flagellata (F-1)
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[Spiro-3]
Sampling site: The Gappei-shori johkasou (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1991)
Culture medium: 1% Agar layer + LE medium, BLEY medium
Food: Bacteria + Flagellata (F-1)
Culture vessel: Petri dish
Temperature: 20 degrees C
Light condition: Dark

[Spiro-4]
Sample: Sludge of the Gappei-shori johkasou (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1991)
Death: 2007.09.19
Culture medium: 1% Agar layer + 1/2LE medium
Food: Bacteria + Flagellata (F-1)
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[Spiro-5]
Sampling site: Second tank of rural sewage treatment plant (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1994.05.11)
Death: 1996.02.19
Culture medium: 1% Agar layer + 1/2LE medium
Food: Bacteria + Flagellata
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Poor growth is shown under the culture conditions, 2-4 cells/petri dish any time.


Stentor sp. [St-1]
Sample: Sludge of the contact aeration tank of soil system in Nagoya city, Aichi Prefecture (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1986.09.25)
Death: 1987.03
Culture medium: LE medium
Food: Bacteria + Flagellata (F-1)
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Body colour is light brown.

[St-2] Stentor coeruleus
Sample: Sludge of the Kohoku Sewage Treatment Plant in Ibaraki Prefecture
Isolated by: Kuniyasu, Yuko (1989.10.20)
Death: 1991.05.08
Culture medium: 1% Agar layer + 1/2LE medium
Food: Bacteria + Tetrahymena pyriformis [Tetra-1]
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Body is blue-coloured.


Tetrahymena pyriformis Ehrenberg [Tetra-1]
Sampling site: The Tuchiura Harbor in Lake Kasumigaura, Ibaraki Prefecture (Sudo, Ryuichi)
Isolated by: Sudo, Ryuichi (1976.08)
Identified by: Sudo, Ryuichi
Culture medium: LE medium, BLEY medium
Food: Bacteria
Culture vessel: Tube or Petri dish (diameter 46 mm)
Temperature: 10, 20 degrees C
Light condition: Dark
Notes: Living in water bloom. Stock in The NIES Culture Center (1987.12 ~)

[Tetra-2]
Sampling site: Kou influent (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1984.07.13)
Culture medium: LE medum
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10, 20 degrees C
Light condition: Dark

[Tetra-3]
Sample: Activated sludge (Sudo, Ryuichi)
Isolated by: Sudo, Ryuichi (1970s)
Culture medium: LE medium, BLEY medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10, 20 degrees C
Light condition: Dark


Trithigmostoma cucullulus [Tri-1]
Sample: Sludge of the contact aeration tank of soil system in Nagoya city, Aichi Prefecture (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1986.07.16)
Death: 1989.05
Culture medium: 10% SE medium
Food: SY-1 and Type 021N
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Subculture frequency is once a week.
References: Journal of Japan Sewage Works Association 23(267):74-86, 1986
24th Japanese Society of Water Treatment Biology (1987)
25th Japanese Society of Water Treatment Biology (1988)

[Tri-2]
Sampling site: Rural sewage treatment plant
Isolated by: Kuniyasu, Yuko (1989.05)
Death: 1991.04
Culture medium: 10% SE medium, 1/2EGG medium + Stigmasterol (1 ul/ml)
Food: T2-1 (Type 021N)
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Subculture frequency is once a week.
References: Ref. ID; 1092
R. Sudo, Y. Inamori, Y. Kuniyasu and T. Ouchiyama: Predation and deodrization musty-odor-producing filamentous Algae by the protozoa Trithigostoma cucullulus. Wat.Sci.Tech. 21(12):1743-1746, 1989
27th Japanese Society of Water Treatment Biology (1990)
24th Japan Water Pollution Research and Control (1990)
25th Japan Water Pollution Research and Control (1991)

[Tri-3]
Sampling site: The contact aeration tank in Teganuma, Chiba Prefecture
Isolated by: Kuniyasu, Yuko (1990.07.09)
Death: 1993.02
Culture medium: 1/2EGG medium + Stigmasterol (1 ul/ml), EGG medium + Stigmasterol (1 ul/ml)
Food: T2-1 (Type 021N)
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Subculture frequency is once a week.
References: Ref. ID; 1337

[Tri-4]
Sampling site: Rural sewage treatment plant
Isolated by: Kuniyasu, Yuko (1991)
Death: 1993.02
Culture medium: 1/2 EGG-medium + Stigmasterol (1 ul/ml), EGG medium + Stigmasterol (1 ul/ml)
Food: T2-1 (Type 021N)
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Subculture frequency is once a week.


Vorticella cupifera [Vor-1]
Sampling site: OD experimental equipment in NIES laboratory (room temperature 20 degrees C)
Isolated by: Kuniyasu, Yuko (1986.03.14)
Death: 1992.12
Identified by: Ouchiyama, Takahiro
Culture medium: LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10 degrees C
Light condition: Dark
Notes: Body length 55 um, body width 37 um, peristome width 18 um; one contractile vacuole; with transverse line; without thecoplasmic granules; macronucleus ribbon-like.

[Vor-2] Vorticella aeguilata
Sampling site: Pond in NIES
Isolated by: Kuniyasu, Yuko (1986.04.14)
Identified by: Ouchiyama, Takahiro
Death: 1990.07
Culture medium: LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10 degrees C
Light condition: Dark
Notes: Body length 100 um, body width 43 um, peristome 38-45 um wide; one contractile vacuole; with clear transverse line, with thecoplasmic granules; macronucleus C-shaped.

[Vor-3]
Sampling site: Rural sewage treatment plant (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1986.12.05)
Death: 1992
Culture medium: 1/2LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10 degrees C
Light condition: Dark
Notes: Prey for Hemiophrys pleurosigma.

[Vor-4] Vorticella cupifera
Sampling site: Rural sewage treatment plant (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1986.12.05)
Identified by: Ouchiyama, Takahiro
Death: 1992.01
Culture medium: 1/2LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10 degrees C
Light condition: Dark
Notes: Body length 55 um, body width 35 um, peristome 20 um wide; one contractile vacuole; with transverse line; without thecoplasmic granules; macronucleus ribbon-like.

[Vor-5]
Sample: Sludge of rural sewage treatment plant (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1986.12.05)
Culture medium: LE medium > BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10, 20 degrees C
Light condition: Dark
Notes: Body length 75 um, body width 37 um, peristome 35 um wide; one contractile vacuole; with transverse line without thecoplasmic granules; macronucleus C-shaped.

[Vor-6]
Sampling site: The aeration tank of the Gappei-shori johkasou (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1987.03.31)
Death: 1989
Culture medium: LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10 degrees C
Light condition: Dark

[Vor-7] Vorticella cupifera
Sampling site: The denitrofication tank of landfill discharge treatment plant in Kashiwa City, Chiba Prefecture (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1987.05.06)
Identified by: Ouchiyama, Takahiro
Death: 2012.03
Culture medium: LE medium, 1/2LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10, 20 degrees C
Light condition: Dark
Notes: Body length 53 um, body width 37 um, peristome 20 um wide; one contractile vacuole; with transverse line; without thecoplasmic granules; macronucleus ribbon-like.

[Vor-8] Vorticella cupifera
Sampling site: Anaerobic filter experimental equipment in NIES laboratory (room temperature 20 degrees C)
Isolated by: Kuniyasu, Yuko (1987.03.30)
Identified by: Ouchiyama, Takahiro
Death: 1992.04.03
Culture medium: LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10 degrees C
Light condition: Dark
Notes: Body length 50 um, body width 37 um, peristome 22 um wide; one contractile vacuole; with transverse line; without thecoplasmic granules.

[Vor-9]
Sampling site: Tandoku-shori johkasou in Kagawa Prefecture (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1988.09.30)
Culture medium: LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10, 20 degrees C
Light condition: Dark

[Vor-10]
Sampling site: The contact aeration tank in Teganuma, Chiba Prefecture
Isolated by: Kuniyasu, Yuko (1989.09.22)
Death: 1990.03
Culture medium: 1/2LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10 degrees C
Light condition: Dark
Notes: Subculture frequency is once a week. Cell size is very big.

[Vor-11]
Sampling site: Gappei-shori johkasou (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1992.06.26)
Death: 1996.03
Culture medium: 1/2LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10 degrees C
Light condition: Dark


Hypotrichida
Sampling site: Johkasou (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1996.02)
Culture medium: BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10 degrees C
Light condition: Dark
Notes: Cell size is very different. Morphogenesis is complex.

Rotifera

BDELLOIDEA

Adineta sp.? [A(R)-12]
Sampling site: The hair dresser's parlor wastewater treatment plant using Gappei-shori johkasou in Misato-mura, Ibaraki Prefecture (Nagasaka)
Isolated by: Kuniyasu, Yuko (1997.02)
Culture medium: 1/2LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark


Habrotrocha sp.?

[H(R)-1]
Sampling site: (Sudo, Ryuichi)
Isolated by: Sudo, Ryuichi (1970s)
Culture medium: LE medium, BLEY medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10, 20 degrees C
Light condition: Dark
Notes: Sudo named Rotaria sp.

[H(R)-2]
Sampling site: (Sudo, Ryuichi)
Isolated by: Sudo, Ryuichi (1970s)
Culture medium: 1/2LE medium, BLEY medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10, 20 degrees C
Light condition: Dark
Notes: Sudo named Rotaria sp.

[H(R)-7]
Sampling site: Night-soil treatment plant in Kurihara City (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1991.12.06)
Culture medium: LE medium >> BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: 10 degrees C is not suitable.

[H(R)-8]
Sampling site: (Sudo, Ryuichi)
Isolated by: Sudo, Ryuichi (1970s)
Culture medium: LE medium, BLEY medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10, 20 degrees C
Light condition: Dark
Notes: Sudo named Rotaria sp.

[H(R)-14]
Sampling site:
Isolated by: Kuniyasu, Yuko
Culture medium: LE medium
Food: Bacteria + Fungi
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark


Philodina erythrophthalma [P-1]
Sampling site: Biofilm of domestic wastewater treatment plant using rotating disc method (Sudo, Ryuichi)
Isolated by: Sudo, Ryuichi (1970s)
Culture medium: LE medium > BLEY medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10, 20 degrees C
Light condition: Dark
Notes: Sudo named P. roseola, but body colour is not red under the culture conditions.
References: Ref. ID; 1063, 1091, 1100, 1108
Journal of Japanese Society of Water Treratment Biology Vol.23(2):15-23 (1987)
Journal of Japan Society on Water Environment Vol.15(7):457-464 (1992)
25th Japan Water Pollution and Research and Control (1991)

[P-3]
Sampling site: No. 1 (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1986.09.26)
Culture medium: BLEY medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Light condition: Dark

[P-4]
Sampling site: Johkasou J-1 (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1988.09.30)
Culture medium: 1/2 LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[P(R)-5]
Sampling site: Tandoku-johkasou H-2 (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1989.06)
Culture medium: LE medium >> BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[P-Kei]
Sampling site: Experimental equipments (No. 2) for wastewater treatment system using common reed in NIES field (Kei)
Isolated by: Kuniyasu, Yuko (2005.06.22)
Culture medium: LE medium >> BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[P-Tanaka]
Sampling site: Johkasou Tanaka 1 (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1996.01.22)
Culture medium: 1/2LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark


Rotaria sp.?

[R-3]
Sampling site: The contact aeration tank in Teganuma, Chiba Prefecture
Isolated by: Kuniyasu, Yuko (1989.10.05)
Death: 1993
Culture medium: 1/2LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[R-4]
Sampling site: The OD wastewater treatment plant in Masuda City (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1989.10.20)
Death: 1996
Culture medium: 1/2LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[R-6]
Sampling site: The contacted aeration tank of Gappei-shori johkasou in Kio city (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1989.11)
Death: 2013
Culture medium: 1% Agar layer + LE medium, 1/2LE medium and BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Cell size was large, spurs were very stout.

[R-9]
Sampling site: Gappei-shori johkasou (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1992.06.26)
Culture medium: 1/2LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[R-11]
Sampling site:
Isolated by: Kuniyasu, Yuko
Culture medium: 1/2LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[R-Kimochi]
Sampling site: Experimental equipment of Mr. Kimochi in NIES laboratory (room temperature 20 degrees C)
Isolated by: Kuniyasu, Yuko (1996.08)
Culture medium: BLEY medium >> LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[R-Shibetu] Rotaria neputunida ?
Sample: Sludge of the Shibetu wastewater treatment system in Shibetu-chou, Hokkaido (Nagasaka)
Isolated by: Kuniyasu, Yuko (2006.09.06)
Culture medium: 1/2LE medium << BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark


MONOGONONTA

Cephalodella sp.?
Sampling site: The Gappei-shori johkasou in Kanazawa City, Ishikawa Prefecture (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1999.03.15)
Culture medium: 1/2LE medium < BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10, 20 degrees C
Light condition: Dark

Colurella sp.
Sample: Sludge of the contact aeration tank of the Gappei-shori johkasou (JARUS IIIR Type) (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1999.01)
Culture medium: BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10, 20 degrees C
Light condition: Dark


Lecane sp. [L-1]
Sample: Activated sludge (Inamori, Yuhei)
Isolated by: Inamori, Yuhei (1980s)
Culture medium: LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10, 20 degrees C
Light condition: Dark

[L-2]
Sample: The river in Oomuta City (Koga)
Isolated by: Kuniyasu, Yuko (1988.03.25)
Culture medium: 1/2LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10, 20 degrees C
Light condition: Dark
Notes: Toe is not separated.

[L-3]
Sampling site: Anaerobic tank in Berlin (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1988.10.21)
Death: 1993
Culture medium: LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[L-4]
Sampling site: Lake Teganuma in Chiba Prefecture
Isolated by: Kuniyasu, Yuko (1989.09.22)
Culture medium: 1/2LE medium
Death: 2009.02.09
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[L-Teru'97]
Sampling site: Johkasou No. 1 (Terunuma)
Isolated by: Kuniyasu, Yuko (1997.07.20)
Culture medium: 1/2LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[L-Teru'00]
Sampling site: Johkasou No. 1 (Terunuma)
Isolated by: Kuniyasu, Yuko (2000.02)
Culture medium: 1/2LE medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[L-Ishi]
Sample: Sludge of the Ishi Wastewater Treatment Center in Ibaraki Prefecture (Nagasaka)
Isolated by: Kuniyasu, Yuko (2006.09.06)
Culture medium: 1/2LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[L-Ginowan]
Sample: Sludge of the Ginowan Wastewater Treatment Center in Okinawa Prefecture (Nagasaka)
Isolated by: Kuniyasu, Yuko (2006.10)
Culture medium: 1/2LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[L-Tairakumou]
Sample: Sludge of the Tairakumou Wastewater Treatment Plant in Kushiro City, Hokkaido (Nagasaka)
Isolated by: Kuniyasu, Yuko (2006.10)
Culture medium: 1/2LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[Mono-1]
Sample: Activated sludge
Isolated by: Inamori, Yuhei? (1980s)
Culture medium: LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 10, 20 degrees C
Light condition: Dark

[Mono-2]
Sample: Sludge of the Toyokawa Wastewater Treatment Center in Toyokawa City, Aichi Prefecture (Nagasaka)
Isolated by: Kuniyasu, Yuko (2006.10)
Culture medium: 1/2LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Male found.


Lepadella sp. [Lep-Kei]
Sampling site: Experimental equipments (No.2) for wastewater treatment system using common reed in NIES field (Kei)
Isolated by: Kuniyasu, Yuko (2005.06.22)
Culture medium: BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[Lep-Kimochi]
Sampling site: The watercourse experimental plant in the Saitama Prefecture Environmental Research Center (Kimochi)
Isolated by: Kuniyasu, Yuko (2003.11.13)
Culture medium: BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Small ciliate are present.

[Lep-Hitachinaka]
Sampling site: The hair dresser's parlor wastewater treatment plant using Gappei-shori johkasou in Hitachinaka City, Ibaraki Prefecture (Nagasaka)
Isolated by: Kuniyasu, Yuko (1997.02)
Culture medium: BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Male found.

[Lepadella]
Sampling site: Indonesia (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1993.11)
Culture medium: BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark
Notes: Small ciliate are present.


Oligochaeta

Aeolosoma hemprichi [Aeolo-1]
Sampling site: Activated sludge of the Ooi Racecourse, In Tokyo (Inamori, Yuhei)
Isolated by: Inamori, Yuhei (1970s)
Culture medium: LE medium >> BLEY medium
Food: Bacteria
Culture vessel: 300 ml elenmyer flask
Temperature: 20 degrees C
Light condition: Dark
References: Ref. ID; 1063, 1091, 1100, 1108, 1208
Journal of Japanese Society of Water Treratment Biology Vol.23(2):15-23 (1987)
18th Japanese Society of Water Treatment Biology (1981)

[Aeolo-2]
Sampling site: (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1989.09.20)
Death: 1990.06
Culture medium: LE medium
Food: Bacteria
Culture vessel: 300 ml elenmyer flask
Temperature: 20 degrees C
Light condition: Dark

[Aeolo-4]
Sampling site: Experimental equipments of Mr. Takai in NIES laboratory (room temperature 20 degrees C)
Isolated by: Kuniyasu, Yuko (1994.02)
Culture medium: 1/2LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[Aeolo-Chikayama]
Sampling site: Johkasou (Chikayama)
Isolated by: Kuniyasu, Yuko (1998.08.05)
Culture medium: LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[Aeolo-Hitachi]
Sampling site: The hair dresser's parlor wastewater treatment plant using the Gappei-shori johkasou in Hitachinaka City, Ibaraki Prefecture (Nagasaka)
Isolated by: Kuniyasu, Yuko (1997.02.06)
Culture medium: LE medium, BLEY medium
Food: Bacteria
Culture vessel: Petri dish (diameter 46 mm)
Temperature: 20 degrees C
Light condition: Dark

[Aeolo-Matue]
Sampling site: The Gappei-shori johkasou treating wastewater from feeding facilities in Matue City, Tottori Prefecture (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1991)
Culture medium: BLEY medium
Food: Bacteria
Culture vessel: 300 ml elenmyer flask
Temperature: 20 degrees C
Light condition: Dark
Notes: This species has not red oil granules, therefore body colour is pale gray. Maximum cell density is very low under the culture conditions.


Dero sp. [Dero-Inamori]
Sampling site: (Inamori, Yuhei)
Isolated by: Inamori, Yuhei (1979)
Death: 1985
Culture medium: SE medium
Food: Bacteria
Culture vessel: 300 ml elenmyer flask
Temperature: 20 degrees C
Light condition: Dark
References: Ref. ID; 1208

[Dero-1]
Sampling site: No. 4 (Inamori, Yuhei)
Isolated by: Kuniyasu, Yuko (1989.11)
Death: 1996
Culture medium: 1/2LE medium
Food: Bacteria
Culture vessel: 300 ml elenmyer flask
Temperature: 20 degrees C
Light condition: Dark
Notes: Activated sludge is necessary for good growth.


Nais sp.
Sampling site: Bio-film treatment plant (Inamori, Yuhei)
Isolated by: Inamori, Yuhei (1970s)
Death: 1985?
Culture medium: SE medium
Food: Bacteria
Culture vessel: 300 ml elenmyer flask
Temperature: 20 degrees C
Light condition: Dark
References: Ref. ID; 1091, 1208

Pristina sp.
Sampling site: Bio-film treatment plant (Inamori, Yuhei)
Isolated by: Inamori, Yuhei (1979)
Death: 1985?
Culture medium: SE medium
Food: Bacteria
Culture vessel: 300 ml elenmyer flask
Temperature: 20 degrees C
Light condition: Dark
References: Ref. ID; 1208