Culture Collection List

Protozoa
Aspidisca sp. Asterocaelum sp. Blephalism sp. Carchesium sp. Colpidium sp. Colpoda sp. Dileptus sp. Epistylis sp. Euplotes sp. Gonostomum sp. Hemiophrys sp. Opercularia sp. Oxytricha sp. Paramecium sp. Pseudoblepharisma sp. Spirostomum sp. Stentor sp. Tetrahymena sp. Trithigmostoma sp. Vorticella sp.

Rotifera
Lecane sp. Monostyla sp. Philodina sp. Rotaria sp.

Oligochaeta
Aeolosoma sp. Dero sp. Nais sp. Pristina sp.

Protozoa

Asterocaelum sp. (Am-1)
Sample: The Yabakei Dam in Ooita prefecture
Isolated by: (Kuniyasu, Yuko)
Culture medium: MA, M11-medium
Food: Anabena (NIES No. 1, No. 77)
Culture Vessel: Petri dish
Temperature: 25 degrees C
Light condition: Approx. 1,000 lux, continuous
Comments: Recommend MA-medium
Reference:

Blephalisma japonicum (Blepha-1)
Sample: OD experimental equipment (In laboratory, 20 degrees C)
Isolated by: 1986. 07. 04 (Kuniyasu, Yuko)
Culture medium: 1/2 LE-medium + trace stigumasterol (50 ul stock solution/50 ml medium)
Food: Bacteria and Flagellata (F-1)
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments: Red coloured
References:

Carchesium sp. (Car-1)
Sample: Rural sewerage system
Isolated by: 1986. 12. 05 (Kuniyasu, Yuko)
Death: 1987. 06
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments: Small colony (< 10 cells in a colony).
References:

Colpidium campylum (Colpi-1)
Sample:
Isolated by: Sudo, Ryuichi
Identificated by: Sudo, Ryuichi
Culture medium: LE-medium, Red pear medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10 degrees C
Comments:
References:

Colpidium sp. (Colpi-2)
Sample: The anaerobic tank of Gappei-shori johkasou
Isolated by: 1987. 04. (Kuniyasu, Yuko)
Culture medium: LE-medium, Red pear medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10 C
Comments:
References:

Colpidium sp.? (Colpi-3)
Sample: Hog wastewater treatment plant
Isolated by: 1992. 06. 26 (Kuniyasu, Yuko)
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Colpoda sp. (Colpo-1)
Sample:
Isolated by: Sudo, Ryuichi
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10 degrees C
Comments: Cyst formation
References:

Colpoda sp. (Colpo-2)
Sample: Anaerobic filter experimental equipment (In laboratory, 20 degrees C)
Isolated by: 1987. 03. 16 (Kuniyasu, Yuko)
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments: Cyst formation. Not suitable 10 degrees C.
References:

Dileptus anser (Dil-1)
Sample: Treatment system of landfill discharge (In Nagoya City)
Isolated by: 1986. 09. 25 (Kuniyasu, Yuko)
Death: 1988. 11
Culture medium: LE-medium
Food: Tetra-1, Colpi-1, Aspi-1
Culture vessel: Petri dish
Temperature: 30 degrees C
Comments: Subculture frequency is once a week. Good growth occurs at high temperatures.
References:

Epistylis sp. (Epi-1)
Sample: The anoxic tank in Berlin
Isolated by: 1988.10.31 (Kuniyasu, Yuko)
Death: 1990.06
Culture medium: 1% Agar layer + LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: Good growth occurs at 20 degrees C. 10 degrees C is not suitable.
Comments:
References:

Epistylis sp. (Epi-2)
Sample: The anaerobic tank in Berlin.
Isolated by: 1988. 10. 31 (Kuniyasu, Yuko)
Death: 1990. 09
Culture medium: 1% Agar layer + LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Euplotes aediculatus (Euplo-1)
Sample: OD experimental equipment (In laboratory, 20 degrees C)
Isolated by: 1986. 03. 28 (Kuniyasu, Yuko)
Death: 1995. 04
Culture medium: LE-medium
Food: Bacteria, Flagellata (F-1)
Culture vessel: Tube
Temperature: 10 degrees C
Comments: Body length 110-120 um, body width 80 um
References:

Euplotes sp. (Euplo-2)
Sample: The Kohoku sewage treatment plant in Ibaraki prefecture.
Isolated by: 1986. 05. 09 (Kuniyasu, Yuko)
Death: 1994. 04
Culture medium: LE-medium
Food: Bacteria, Flagellata (F-1)
Culture vessel: Tube
Temperature: 10 degrees C
Comments:
References:

Euplotes sp. (Euplo-3)
Sample: The aerobic tank of Gappei-shori johkasou.
Isolated by: 1987. 04. (Kuniyasu, Yuko)
Death: 1988.
Culture medium: 1/2 LE-medium
Food: Bacteria, Flagellata (F-1)
Culture vessel: Petri dish
Temperature:
Comments:
References:

Euplotes sp. (Euplo-4)
Sample: The anaerobic contacted filter experimental equipment (In laboratory, 20 degrees C)
Isolated by: 1987. 03. 30 (Kuniyasu, Yuko)
Death: 1996. 04. 22
Culture medium: LE-medium
Food: Bacteria, Flagellata (F-1)
Culture vessel: Tube
Temperature: 10 degrees C
Comments:
Refereneces:

Euplotes sp. (Euplo-5)
Sample: OD experimental equipment (In laboratory, 20 degrees C)
Isolated by: 1991. 12. 06 (Kuniyasu, Yuko)
Death: 1994. 09
Culture medium: 1/2 LE-medium
Food: Bacteria, Flagellata (F-1)
Culture vessel: Petri dish
Temperetaure: 20 degrees C
Comments:
References:

Gonostomum sp. (Gono-1)
Sample: The pond in NIES
Isolated by: 1986. 04. 28 (Kuniyasu, Yuko)
Death: 1990. 06
Culture medium: 1/2 LE-medium
Food: Bacteria, Flagellata (F-1)
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Hemiophrys pleurosigma
Sample: Rural sewerage system
Isolated by: 1986. 12. 05 (Kuniyasu, Yuko)
Death: 1987. 06
Identified by: Ouchiyama Takahiro
Culture medium: 1/2 LE-medium
Food: Vorticella sp. (Vor-3) + Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Opercularia sp. (Ope-1)
Sample:
Isolated by: (Sudo, Ryuichi)
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments:
References:

Oxytricha fallax (Oxy-1)
Sample: The contacted aeration experimental equipment. (In laboratory, 20 degrees C)
Isolated by: 1986. 06. 27 (Kuniyasu, Yuko)
Death: 1994
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments: Good growth occurs at 20 degrees C. 10 degrees is not suitable.
References:

Oxytricha sp. (Oxy-2)
Sample: The aeration tank of Gappei-shori johkasou.
Isolated by: 1987. 04. 06 (Kuniyasu, Yuko)
Death: 1994.
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments: Good growth occurs at 20 degrees C. 10 degrees is not suitable.
References:

Oxytricha sp. (Oxy-3)
Sample: The river in Oomuta city (from Mis. Koga)
Isolated by: 1988. 03. 25 (Kuniyasu, Yuko)
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments: Good growth occurs at 20 degrees C. 10 degrees is not suitable.
References:

Paramecium aurelia (Para-1)
Sample: OD experimental equipment (In laboratory, 20 degrees C)
Isolated by: 1986. 04. 04 (Kuniyasu, Yuko)
Death: 1990
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10 degrees C
Comments:
References:

Paramecium bursalia (Para-2)
Sample: Ditch in NIES
Isolated by: 1986. 04. 09 (Kuniyasu, Yuko)
Death: 1988. 12
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Light condition: Approx. 1,500 lux, continuous
Comments:
References:

Paramecium caudatum (Ehrenberg)
Sample: Pond at side green-house in NIES (Kuniyasu, Yuko)
Isolated by: 1984. 04. 25 (Kuniyasu, Yuko)
Death: 1990?
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Paramecium sp. (Para-3)
Sample: The anaerobic tank of Gappei-shori johkasou
Isolated by: 1987. 03. 31 (Kuniyasu, Yuko)
Death: 1994. 09.
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10 degrees C
Comments:
References:

Paramecium sp. (Para-4)
Sample: The treatment plant of Nagaoka Sangyo.
Isolated by: 1986. 12. 05 (Kuniyasu, Yuko)
Death: 1990
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10 degrees C
Comments:
References:

Paramecium sp. (Para-5)
Sample: Rural sewerage system
Isolated by: 1986. 12. 05 (Kuniyasu, Yuko)
Death: 1996. 09
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10 degrees C
Comments:
References:

Paramecium sp. (Para-6)
Sample: The anaerobic filter experimental equipment. (In laboratory, 20 degrees C)
Isolated by: 1987. 03. 30 (Kuniyasu, Yuko)
Death: 1988. 08. 24
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Tube or petri dish
Temperature:
Comments:
References:

Paramecium polycarum Woodruff & Spencer (Para-7)
Sample: The Mikawashima wastewater treatment plant in Tokyo. Activated sludge process
Isolated by: 1968. 10 (Sudo, Ryuichi)
Death: 1989. 10
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10 degrees C
Comments:
References:

Paramecium sp. (Para-8)
Sample: The pond in NIES
Isolated by: 1992. (Kuniyasu, Yuko)
Culture medium: 1/2 LE-medium
Food: Bacteria, Algae
Culture vessel: Petri dish
Temperature: 25 degrees C
Light condition: Approx. 1,000 lux, continuous
Comments:
References:

Pseudoblepharisma tenuis (Pblepha-1)
Sample: The aeration tank of Gappei-shori johkasou
Isolated by: 1987. 04. 09 (Kuniyasu, Yuko)
Dead: 1997. 03. 10
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments: Body is red-coloured.
References:

Spirostomum teres Claparede & Lachmann (Spiro-1)
Sample: The Gappei-shori johkasou in Yachiyo city in Chiba prefecture.
Isolated by: 1984. 06. 14 (Kuniyasu, Yuko)
Death: 1990
Culture medium: 1% Agar layer + 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Spirostomum sp. (Spiro-2)
Sample: The Gappei-shori johkasou
Isolated by: 1991. 06. 18 (Kuniyasu, Yuko)
Death: 1994. 09
Culture medium: 1% Agar layer + 1/2 LE-medium
Food: Bacteria, Flagellata (F-1)
Culture vessel: Petri dish
Temperature 20 degrees C
Comments:
References:

Spirostmum sp. (Spiro-3)
Sample: The Gappei-shori johkasou
Isolated by: 1991. (Kuniyasu, Yuko)
Culture medium: 1% Agar layer + 1/2 LE-medium
Food: Bacteria, Flagellata (F-1)
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Spirostmum sp. (Spiro-4)
Sample: The Gappei-shori johkasou
Isolated by: 1991 (Kuniyasu, Yuko)
Death: 2007. 09. 19
Culture medium: 1% Agar layer + 1/2 LE-medium
Food: Bacteria, Flagellata (F-1)
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Spirostmum sp. (Spiro-5)
Sample: The second tank of rural sewerage system.
Isolated by: 1994. 05. 11 (Kuniyasu, Yuko)
Death: 1996. 02. 19
Culture medium: 1% Agar layer + 1/2 LE-medium
Food: Bacteria, Flagellata
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments: Poor growth is shown under these conditions. Almost 2-4 cells/petri dish.
References:

Stentor sp. (St-1)
Sample: The sampled aeration tank of soil system in Nagoya city in Nagoya prefecture.
Isolated by: 1986. 09. 25 (Kuniyasu, Yuko)
Death: 1987. 03
Culture medium: LE-medium
Food: Bacteria, Flagellata (F-1)
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Stentor coeruleus (St-2)
Sample: The Kohoku wastewater treatment plant in Ibaraki prefecture.
Isolated by: 1989. 10 (Kuniyasu, Yuko)
Death: 1991. 05. 08
Culture medium: 1% Agar layer + 1/2 LE-medium
Food: Bacteria, Tetrahymena pyriformis (Tetra-1)
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments: Body is blue-coloured.
References:

Tetrahymena pyriformis Ehrenberg (Tetra-1)
Sample: The Tuchiura Harbor in Lake Kasumigaura in Ibaraki prefecture.
Isolated by: 1976. 08. (Sudo, Ryuichi)
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Tube, Petri dish
Temperature: 10 degrees C
Comments: Living in water bloom. Stock in The NIES Culture Center (1987. 12 ~)
References:

Tetrahymena sp. (Tetra-2)
Sample: Kou influent (Inamori, Yuhei)
Isolated by: 1984. 07. 13 (Kuniyasu, Yuko)
Culture medium: LE-medum
Food: Bacteria
Culture vessel: Tube
Temperature: 10 degrees C
Comments:
References:

Tetrahymena sp. (Tetra-3)
Sample: Activated sludge
Isolated by: (Sudo, Ryuichi)
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10 degrees C
Comments:
References:

Trithigmostoma cucullulus (Tri-1)
Sample: The sampled aeration tank of soil system in Nagoya city in Nagoya prefecture.
Isolated by: 1986. 07. 16 (Kuniyasu, Yuko)
Death: 1989. 05
Culture medium: 10% SE-medium
Food: SY-1, Type 021N
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Trithigmostoma cucullulus (Tri-2)
Sample: Rural sewerage system.
Isolated by: 1989. 05 (Kuniyasu, Yuko)
Death: 1994. 04
Culture medium: 10% SE-medium, 1/2 or 1/4 EGG-medium + Stigmasterol (1 ul/ml)
Food: T2-1 (Type 021N)
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Trithigmostoma sp. (Tri-4)
Sample: Rural sewerage system.
Isolated by: 1991. (Kuniyasu, Yuko)
Death: 1994. 12.
Culture medium: 1/2 EGG-medium + Stigmasterol (1 ul/ml)
Food: T2-1 (Type 021N)
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Vorticella cupifera (Vor-1)
Sample: OD experimental equipment. (In laboratory, 20 degrees C)
Isolated by: 1986. 03. 14 (Kuniyasu, Yuko)
Death: 1993.
Identified by: Ouchiyama, Takahiro
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments: Body length 55 um, body width 37 um, peristome width 18 um; one contractile vacuole; with transverse line; without thecoplasmic granules; macronucleus ribbon-like.
References:

Vorticella aeguilata (Vor-2)
Sample: The pond in NIES
Isolated by: 1986. 04. 14 (Kuniyasu, Yuko)
Identified by: Ouchiyama, Takahiro
Death: 1990. 07
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments: Body length 100 um, body width 43 um, peristome 38-45 um wide; one contractile vacuole; with clear transverse line, with thecoplasmic granules; macronucleus C-shaped.
References:

Vorticella sp. (Vor-3)
Sample: Rural sewerage system.
Isolated by: 1986. 12. 05 (Kuniyasu, Yuko)
Death: 1990?
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments:
References:

Vorticella cupifera (Vor-4)
Sample: Rural sewerage system.
Isolated by: 1986. 12. 05 (Kuniyasu, Yuko)
Death: 1990?
Identified by: Ouchiyama, Takahiro
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments: Body length 55 um, body width 35 um, peristome 20 um wide; one contractile vacuole; with transverse line; without thecoplasmic granules; macronucleus ribbon-like.
References:

Vorticella sp. (Vor-5)
Sample: Rural sewerage system.
Isolated by: 1986. 12. 05 (Kuniyasu, Yuko)
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments: Body length 75 um, body width 37 um, peristome 35 um wide; one contractile vacuole; with transverse line without thecoplasmic granules; macronucleus C-shaped.
References:

Vorticella sp. (Vor-6)
Sample: The aeration tank of Gappei-shori johkasou.
Isolated by: 1987. 03. 31 (Kuniyasu, Yuko)
Death: 1989
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments:
References:

Vorticella cupifera (Vor-7)
Sample: The denitrofication tank of landfill discharge treatment plant in Kashiwa city in Chiba prefecture.
Isolated by: 1987. 05. 06 (Kuniyasu, Yuko)
Identified by: Ouchiyama, Takahiro
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments: Body length 53 um, body width 37 um, peristome 20 um wide; one contractile vacuole; with transverse line; without thecoplasmic granules; macronucleus ribbon-like.
References:

Vorticella cupifera (Vor-8)
Sample: Anaerobic filter experimental equipment. (In laboratory, 20 degrees C)
Isolated by: 1987. 03. 30 (Kuniyasu, Yuko)
Identified by: Ouchiyama, Takahiro
Death: 1993
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments: Body length 50 um, body width 37 um, peristome 22 um wide; one contractile vacuole; with transverse line; without thecoplasmic granules;

Vorticella sp. (Vor-9)
Sample: Tandoku-shori johkasou in Kagawa prefecture.
Isolated by: 1988. 09. 30 (Kuniyasu, Yuko)
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments:
References:

Vorticella sp. (Vor-10)
Sample: Lake Teganuma in Chiba prefecture.
Isolated by: 1989. 09. 22 (Kuniyasu, Yuko)
Death: 1990. 01
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments: Subculture frequency is once a week.
References:

Vorticella sp. (Vor-11)
Sample: Gappei-shori johkasou
Isolated by: 1992. 06. 26 (Kuniyasu, Yuko)
Death: 1996. 3
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments:
References:

Rotifera

Philodina sp. (P-2)
Sample:
Isolated by: (Kuniyasu, Yuko)
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments:
References:

Philodina sp. (P-3)
Sample: No. 1 (Inamori, Yuhei)
Isolated by: 1986. 09. 26 (Kuniyasu, Yuko)
Culture medium: 1% Agar layer + LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Philodina sp. (P-4)
Sample: Johkasou (J-1)
Isolated by: 1988. 09. 30 (Kuniyasu, Yuko)
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments: Colony formation?
References:

Rotaria sp. (R-1)
Sample:
Isolated by: Sudo, Ryuichi
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10 degrees C
Comments:
References:

Rotaria sp. (R-2)
Sample:
Isolated by: (Sudo, Ryuichi)
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10 degrees C
Comments:
References:

Rotaria sp. (R-3)
Sample: Lake Teganuma in Chiba prefecture.
Isolated by: 1989. 10. 05 (Kuniyasu, Yuko)
Death: 1993
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Rotaria sp. (R-4)
Sample: OD in Masuda (Inamori, Yuhei)
Isolated by: 1989. 10. 20 (Kuniyasu, Yuko)
Death: 1993
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Rotaria sp. (R-5)
Sample: Tandoku-johkasou (H-2)
Isolated by: 1989. 06. (Kuniyasu, Yuko)
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Rotaria sp. (R-6)
Sample: The contacted aeration tank of Gappei-shori johkasou in Kio city.
Isolated by: 1989. 11. (Kuniyasu, Yuko)
Culture medium: 1% Agar layer + 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Rotaria sp. (R-7)
Sample: Night-soil treatment plant in Kurihara.
Isolated by: 1991. 12. 06 (Kuniyasu, Yuko)
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments: 10 degrees C is not suitable.
References:

Rotaria sp. (R-8)
Sample:
Isolated by: (Sudo, Ryuichi)
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Tube
Temperature: 10 degrees C
Comments:
References:

Rotaria sp. (R-9)
Sample: Gappei-shori johkasou
Isolated by: 1992. 06. 26 (Kuniyasu, Yuko)
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments: 10 degrees C is not suitable.
References:

Lecane sp. (L-1)
Sample:
Isolated by: (Kuniyasu, Yuko)
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments:
References:

Lecane sp. (L-2)
Sample: The river in Oomuta city (from Mis. Koga)
Isolated by: 1988. 03. 25 (Kuniyasu, Yuko)
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments:
References:

Lecane sp. (L-3)
Sample: The Anaerobic tank in Berlin.
Isolated by: 1988. 10. 26 (Kuniyasu, Yuko)
Death: 1993
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments:
References:

Lecane sp. (L-4)
Sample: Lake Teganuma in Chiba prefecture.
Isolated by: 1989. 09. 22 (Kuniyasu, Yuko)
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 20 degrees C
Comments:
References:

Monostyla sp. (Mono-1)
Sample:
Isolated by: (Kuniyasu, Yuko)
Culture medium: LE-medium
Food: Bacteria
Culture vessel: Petri dish
Temperature: 10 degrees C
Comments:
References:

Oligochaeta

Aeolosoma sp. (Aeolo-2)
Sample: (Inamori, Yuhei)
Isolated by: 1989. 09. (Kuniyasu, Yuko)
Death: 1990. 06
Culture medium: LE-medium
Food: Bacteria
Culture vessel: 300 ml flask
Temperature: 20 degrees C
Comments:
References:

Dero sp. (Dero-1)
Sample: No. 4 (Inamori, Yuhei)
Isolated by: 1989. 11. (Kuniyasu, Yuko)
Death: 2000?
Culture medium: 1/2 LE-medium
Food: Bacteria
Culture vessel: 300 ml flask
Temperature: 20 degrees C
Comments:
References:

Nais sp.
Sample: Bio-film treatment plant.
Isolated by: ? (Inamori, Yuhei)
Death: 1985?
Culture medium: Sterilized sludge.
Food: Bacteria
Culture vessel: 300 ml flask.
Temperature: 20 degrees C
Comments:
References:

Pristina sp.
Sample: Bio-film treatment plant.
Isolated by: ? (Inamori, Yuhei)
Death: 1985?
Culture medium: Sterilized sludge.
Food: Bacteria
Culture vessel: 300 ml flask
Temperature: 20 degrees C
Comments:
References: