Ref ID : 950
Barry S. Fields, Thomas A. Nerad, Thomas K. Sawyer, C. Harold King, James M. Barbaree, William T. Martin, William E. Morrill, and Gary N. Sanden; Characterization of an axenic strain of Hartmannella vermiformis obtained from an investigation of Nosocomial Legionellosis. J.Protozool. 37(6):581-583, 1990
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A free-living amoeba identified as Hartmannella vermiformis was isolated from a water sample obtained during an investigation of nosocomidal legionellosis. Hartmannella vermiformis is known to support the intracellular multiplication of Legionella pneumophila. This strain of H. vermiformis, designated CDC-19, was cloned and established in axenic culture to develop a model for the study of the pathogenicity of legionellae. Isoenzyme patterns of axenically-cultivated strain CDC-19 were compared with two strains of H. vermiformis derived from the type strain, one axenic (ATCC50236) and the other grown in the presence of bacteria (ATCC30996). Enzyme patterns suggested that all three strains are assignable to the species H. vermifromis. Axenic H. vermiformis strain CDC-19 has been deposited with the American Type Culture Collection (ATCC50237) and should prove useful in the study of protozoan-bacterial interaction.