Ref ID : 887
S.L. Josephson, R.R. Weik, and D.T. John; Concanavalin A-induced agglutination of Naegleria. American J.Tropical Medicine and Hygiene 26(5):856-858, 1977
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Concanavalin A (Con A) agglutinated all Naegleria gruberi strains tested but did not agglutinate any N. fowleri strains tested. Agglutination was time and temperature dependent and Con A concentration and amoeba concentration dependent over certain ranges. Agglutination increased to maximum up to 1 hr. incubation with Con A. At least 1x10E6 amoebae/ml were needed for maximum agglutination, and Con A concentrations higher than 100 ug/ml did not appreciably increase agglutination. Incubation at 4 degrees C of with 10 mM alpha-methyl-D-mannoside inhibited agglutination of N. gruberi. These data indicate a difference in polysaccharide structure of cell membranes of N. fowleri and N. gruberi.