Ref ID : 839
Ora Hadas, Yehuda Kott, Uriel Bachrach, and Benzion Cavari; Ability of Daphnia cell-free extract to damage Escherichia coli cells. Appl.Environ.Microbiol. 45(4):1242-1246, 1983
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A cell-free extract of Daphnia magna was found to lyse Escherichia coli cells as shown by leakage of the enzymes alkaline phosphatase and beta-galactosidase from the bacteria. The cell-free extract was separated on Sephadex G-200, and the fractions showing an ability to lyse E. coli cells were isolated. The factor which was responsible for the lysis of the bacterial cells was probably a protein with a molecular weight of several thousands. Mg2+ and Ca2+ ions augmented the activity of the Daphnia extract on E. coli cells.