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The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 147

Reuter, G., Aumann, J., Wyss, U., Jansson, H.B., and Schauer, R.; Panagrellus redivivus: failure to find evidence for the occurrence and biosynthesis of sialic acids. Exp.Parasitol. 73:389-395, 1991

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The occurrence of sialic acids in the free-living nematode Panagrellus redivivus was studied by periodate oxidation/[3H]sodium borohydride reduction of about 10(7) nematodes. In parallel, the capability of sialic acid biosynthesis was examined by metabolic labeling of the same number of nematodes with N-[3H]acetylmannosamine. In both experiments, radioactivity was incorporated into the nematodes. Mild acid hydrolysis, however, did not release radioactively labeled sialic acids or derivatives as tested by radio thin-layer chromatography, suggesting that P. redivivus does not contain or synthesize sialic acids.