Ref ID : 7398
Noguchi Munenori, Inoue Hiroshi, Atago Toshihiro, and Nakamura Shogo; Effects of Organic Solvents on the Coupling Between ATP Hydrolysis and Sliding of Microtubules in Axonemes from Chlamydomonas Flagella. J.Eukaryot.Microbiol. 44(2):127-133, 1997
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The effects of organic solvents on the ATPase activity and the sliding disintegration of axonemes from Chlamydomonas were investigated. The axonemal ATPase was markedly activated by methanol accompanying with marked inhibition of the sliding disintegration of axonemes. On the contrary, glycerol inhibited the ATPase activity without serious inhibition of the sliding disintegration. As far as the axonemes are not irreversibly denatured by extremely high concentration of solvents, the effects of solvents both on the ATPase and the ability of sliding are reversible. Therefore, the inhibition of sliding accompanied by the activation of ATPase is probably due to an inability to couple the hydrolysis of ATP to sliding between dynein and microtubule in the presence of methanol. The axonemal ATPase was less sensitive to vanadate inhibition after exposure to methanol. This indicates that methanol makes the dynein-ADP-Pi complex unstable and increases product release. On the other hand, glycerol and ethylene glycol seem to stabilize the force generation resposible for the sliding through stabilizing the dynein-ADP-Pi complex.