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The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 7060

Daniel Dive, S. Robert, E. Angrand, C. Bel, H. Bonnemain, L. Brun, Y. Demarque, A. Le Du, R. El Bouhouti, M.N. Fourmaux, L. Guery, O. Hanssens, and M. Murat; A bioassay using the measurement of the growth inhibition of a ciliate protozoan: Colpidium campylum Stokes. Hydrobiologia 188/189:181-188, 1989

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A bioassay method using the ciliate protozoan Colpidium campylum is presented in a standardized form. The influence of the initial cell concentration on the potassium dichromate EC50 values was determined. Two intercalibration experiments between two laboratories were performed on ten toxicants in two different conditions. The potassium dichromate EC50 determinations performed by eight different people are also presented. All results are discussed in terms of feasibility and reproducibility of the method, fields of application, and limitations.