Ref ID : 5931
Christopher G. Kreis and John D. Stubbs; Presence of DNA nuclear membrane complex in Tetrahymena pyriformis. Can.J.Zool. 56:192-200, 1978
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A DNA-membrane complex enriched in newly synthesized DNA has been isolated from synchronized Tetrahymena pyriformis GL macronuclei. The complexes are formed in the presence of the detergent sodium lauroyl sarcosinate and most of the DNA and membrane of the macronuclei. Shearing of the complex is sufficient to release most of the bulk DNA. However, 90% of newly synthesized DNA remains tightly bound to the complex. It can be shown by pulse-chase experiments that this newly synthesized DNA can be chased from the complex, thus indicating that this DNA is only transiently associated with the complex. The complex appears to be cell cycle specific since it can only be isolated from cells in the S period. Chromatin or purified DNA from log or stationary phase cells does not form a complex when mixed with the detergent and disrupted macronuclei. It appears from these data that the DNA growing points may initiate at the membrane-DNA junction.