Ref ID : 4772
Roman Janisch; Immunofluorescence Detection of Microtubular Structures in Paramecium caudatum by Means of Monoclonal Antibody TU-01. Arch.Protistenk 137:309-316, 1989
Reprint
In File
Notes
Microtubular structures of the cortical cytoskeleton in Paramecium caudatum were identified by means of indirect immunofluorescence employing the monoclonal antibody TU-01. Specimens for observation were fixed with glutaraldehyde in a layer attached to a slide or made into a suspension and without fixation, permeabilized with Triton X-100. The antibody TU-01 reacted with kinetosomes, bundles of postoral microtubules and microtubules of somatic cilia along their whole length. The cells on a slide were well preserved though often deformed in shape and poorly extracted. The cells in suspension, on the other hand, were better extracted but about 10% of them became disrupted. The resultant fragments were used with advantage for studying organization of microtubular structures. Immunofluorescence labelling with TU-01 is a useful tool in the study of the cortical cytoskeleton in, for instance, morphological mutants or abnormal cells of paramecia, and in investigations into the behaviour of the cytoskeleton during repair processes occurring in mechanically injured cells.