Ref ID : 4549
M.V. Rossetti, A.A. Juknat, and A.M.del C. Batlle; Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis. Z.Naturforsch. 44c:578-580, 1989
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A highly efficient method employing NaSCN as a chaotropic agent was used to dissociate the membrane-bound porphobilinogen-deaminase. The same sequence of steps was applied for purifying both soluble and membrane dissociated porphobilinogen-deaminase. The chromatographic behaviour of both proteins was quite similar. Euglena gracilis deaminase appears to exist in an equilibrium mixture of two active species of relative molecular masses of 40000 and 20000.