Ref ID : 4545
A.A. Juknat, D. Dornemann, and H. Senger; Biosynthesis of Porphyrinogens in Etiolated Euglena gracilis Z. I. Isolation and Purification of an Endogenous Factor Stimulating the Formation of Porphyrinogens. Z.Naturforsch. 43c:351-356, 1988
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A low molecular weight, heat-stable factor has been purified from Euglena gracilis supernatant fraction by employing gel filtration, cation and anion exchange and paper chromatography. This endogenous compound stimulates porphobilinogenase (PBG-ase) (E.C. 4.3.1.8) activity, an enzyme of the porphyrin biosynthesis pathway. 10E-7 M folic acid and 10E-4 M 6-biopterin produced a significant activation, equivalent to 2-4 units of the purified factor. Elution patterns from the columns and fluorescence and UV absorption peaks suggest that this compound is a pteridine. This conclusion is further supported by the fact that both, folic acid and 6-biopterin can replace the action of the isolated factor on PBG-ase. The mechanism of stimulation is discussed.