Ref ID : 4157
Ruben Lopez-Revilla and Rodolfo Gomez-Dominguez; Incorporation and Toxicity of 32P-Orthophosphate and Occurrence of Polyphosphate in Entamoeba Trophozoites. J.Protozool. 32(2):353-355, 1985
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We explored the requirements of inorganic phosphate (Pi), the incorporation of 32P-orthophosphate (32Pi), and the occurrence of inorganic polyphosphate (polyP) in axenic Entamoeba cultures. Maximal population densities and growth rates of Entamoeba histolytica trophozoites were attained in complete TP-S-1 medium. As 32Pi concentration was increased in the medium, its own incorporation and the culture growth rate were progressively inhibited, especially in Pi-deficient medium. PolyP grains were found in the cytoplasm and occasionally in the nuclear membrane of E. histolytica, E. histolytica-like, E. invadens, and E. moshkovskii trophozoites.