Ref ID : 3909
R. Neal Band and Helen Cirrito; Growth Response of Axenic Entamoeba histolytica to Hydrogen, Carbon Dioxide, and Oxygen. J.Protozool. 26(2):282-286, 1979
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Entamoeba histolytica required CO2 for growth in axenic culture while growth was inhibited by H2. The organism was tolerant to 5% O2 in the gas phase and it was able to detoxify products of O2 reduction in the medium. The ameba did not require a negative oxidation-reduction potential for axenic growth. However, little or no free O2 was present in media exposed to 5% O2 in the gas phase. Growth was improved by adding yeast extract to the medium.