Ref ID : 3863
G. Wesley Vick,III and Jacob J. Blum; Concanavalin A-Sepharose Chromatography of Hexosaminidase Isozymes Secreted by Tetrahymena. J.Protozool. 27(2):248-249, 1980
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Tetrahymena pyriformis strain HSM secretes 4 isozymes of hexosaminidase. Purified isozymes B1 and B2 are eluted from the void volume of a concanavalin A-Sepharose column, suggesting that they are not glycosylated. Purified isozymes A1 and A2 bind to the column and are eluted at ~0.1 M alpha-methylmannoside, suggesting that these isozymes are glycoproteins. In agreement with earlier deductions based on a differential kinetic assay for the A and B isozymes, the elution pattern of hexosaminidase activity from material secreted by cells grown to early and late stationary phase was consistent with these secretions containing primarily the B and the A isozymes, respectively.