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The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 3823

Christine Ko and Lester Goldstein; A Method for the Electrophoretic Analysis of Proteins and RNAs of Single Cells. J.Protozool. 25(2):261-264, 1978

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A method is described for the electrophoretic analysis of proteins or RNAs from individuals amebae. The method is based on fluorographic autoradiography of semi-micro polyacrylamide gels in which [35S]methionine or [3H]uridine materials from single cells have been subjected to electrophoresis. The method is more sensitive and provides better resolution than previous methods for single cells. It is suitable, also, for quantitation of the separated components.