Ref ID : 3713
Jacques Bohatier; [Syntheses Macromoleculaires au Cours de la Morphogenese de Regeneration chez le Cilie Condylostoma magnum: Action de Differents Inhibiteurs I. Syntheses d'Acides Nucleiques] (Macromolecular Syntheses during Oral Regeneration in the Ciliate Condylostoma magnum: Effect of Different Inhibitors I. Nucleic Acids Synthesis). Arch.Protistenk 124:144-172, 1981
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Traumatic regeneration of the oral apparatus in the ciliate Condylostoma magnum is an example of unicellular morphogenetic development. Nuclear systheses essential to regeneration were studied by use of 3 specific inhibitors of DNA or RNA synthesis (Mitomycin C, Actinomycin D and alpha-amanitin). Postperistomial fragments were exposed to the inhibitors immediately or at various times after cutting; then protargol-impregnated preparations were studied with light microscope. The results show that oral regeneration in C. magnum is independant of DNA synthesis, but initiates actinomycin-sensitive (and alpha-amanitin-sensitive) DNA-templated RNA synthesis. Applications of Actinomycin D during the course of morphogenesis suggest that new RNA are successively required for the all steeps of oral regeneration, that is to say 1) Kinetosome proliferation; 2) adoral and 3) paroral ciliature differentiation; 4) invagination of feeding organelles; 5) formation of food vacuoles. The rate of RNA synthesis (studied by incorporation of [3H]-uridine) is very low, and it occurs the highest during the first hour. These results are discussed in relation to different conclusions given about other ciliates.