Ref ID : 3436
Yu.G. Geltzer; [Methods of studying soil protozoa]. Akademy of Sciences of the USSR Society of Protozoologists of the USSR Series << Protozoology >> 5:154-165, 1980
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Critical analysis of practical laboratory methods of soil protozoa isolation, identification and estimation has been given. Estimation methods, including statistical treatment of the results, are described in detail. Special tables (McCrady and Fisher's) are given for the assessment of cell amounts per 1 g of soil. The total amount of cells is obtained as a sum of the number of every species. Certain nutrient media, conforming the specifity of protozoa groups, make it possible to isolate the greatest amount of cells. Special methods of revealing the interrelation between protozoa and soil microflora and between protozoa and higher plants are proposed. Available cytological reactions are described, used to distinguish between definite cell organoids essential for identification.