Latest Update：2025/12/18 17:05:45

Aoki Masataka

Researcher Name: Aoki Masataka

Div (Section) name/title: Regional Environment Conservation Division（Environment Management and Technology Section）／Researcher

Research Subject: Hygienic assessment of water environment and water conservation technologies

Degree(s): Dr. Eng.

Professional Qualification(s): Civil Engineering,Biology,Bioengineering

Environmental Speciality Field: microbial ecology,environmental microbiology,environmental technology

Keyword(s): microbial community structure analysis,microbial cultivation,biological wastewater treatment

Career: Jun 2021-Present: Researcher (Tenure-track), Regional Environment Conservation Division, National Institute for Environmental Studies (NIES)
Jan 2020-May 2021: Associate Professor, Department of Civil Engineering, National Institute of Technology (NIT), Wakayama College
Apr 2018-Dec 2019: Junior Associate Professor, Department of Civil Engineering, NIT, Wakayama College
Nov 2015-Mar 2018: Assistant Professor, Department of Civil Engineering, NIT, Wakayama College
Sep 2011-Mar 2016: Research Student, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
Feb 2015-Oct 2015: Visiting Student Researcher, California Institute of Technology (Caltech)
Apr 2013-Oct 2015: Japan Society for the Promotion of Science (JSPS) Research Fellow (DC1)

URL: https://researchmap.jp/m-aoki?lang=en
https://www.researchgate.net/profile/Masataka-Aoki
https://orcid.org/0000-0002-9352-2072

Membership of Academic Society: Japan Society on Water Environment (JSWE),The Japanese Society of Microbial Ecology (JSME)

Research Subject

Fiscal Year: 2025
- 27222 : Proposal and evaluation of environmentally efficient technologies and systems in collaboration with local communities
- 27247 : Regional Environment Conservation Domain: Foresight and Advanced Basic Research
- 27281 : Development of highly efficient methanogenic wastewater treatment technology utilizing direct electron transfer
- 27347 : Development of a cultivation method for manganese-oxidizing microorganisms using woody biomass and its application to metal recovery
- 27348 : Development of a new cultivation strategy for aerobic denitrifying microbial dark matter
- 27377 : Development of a gene quantification method for rapid hygienic water quality assessment in disaster situations
Fiscal Year: 2024
- 26837 : Proposal and evaluation of environmentally efficient technologies and systems in collaboration with local communities
- 26864 : Regional Environment Conservation Domain: Foresight and Advanced Basic Research
- 26954 : Development of a cultivation method for manganese-oxidizing microorganisms using woody biomass and its application to metal recovery
- 26955 : Development of a gene quantification method for rapid hygienic water quality assessment in disaster situations
- 27167 : Development of a new cultivation strategy for aerobic denitrifying microbial dark matter
Fiscal Year: 2023
- 26426 : Proposal and evaluation of environmentally efficient technologies and systems in collaboration with local communities
- 26453 : Regional Environment Conservation Domain: Foresight and Advanced Basic Research
- 26509 : Evaluation of the fate of pathogenic bacteria with a view to reducing hygiene risk and development and implementation of appropriate wastewater treatment technology
- 26515 : Development of a cultivation method for manganese-oxidizing microorganisms using woody biomass and its application to metal recovery
Fiscal Year: 2022
- 26010 : Regional Environment Conservation Domain: Foresight and Advanced Basic Research
- 26084 : Evaluation of the fate of pathogenic bacteria with a view to reducing hygiene risk and development and implementation of appropriate wastewater treatment technology
- 26085 : Development of a cultivation method for Mn-oxidizing bacteria and a minor metal recovery method using biodegradable plastics
Fiscal Year: 2021
- 25621 : Evaluation of the fate of pathogenic bacteria with a view to reducing hygiene risk and development and implementation of appropriate wastewater treatment technology
- 25629 : Development of a cultivation method for Mn-oxidizing bacteria and a minor metal recovery method using biodegradable plastics
- 25713 : Regional Environment Conservation Domain: Foresight and Advanced Basic Research

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Research Result (Publication)

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Original Paper

Original Paper
A biofilm reactor packed with aspen wood and polyurethane sponge for biological manganese(II) oxidation and minor metal recovery

Presenter : Aoki M., Hayashi K., Wakui N., Hirakata Y., Watari T., Yamaguchi T., Syutsubo K.
Title : , World Journal of Microbiology and Biotechnology, 41(12):491 (2025)
Original Paper
Downflow sponge biofilm reactors for polluted raw water treatment: Performance optimisation, kinetics, and microbial community

Presenter : Loi J.X., Syutsubo K., Rabuni M.F., Takemura Y., Aoki M., Chua A.S.M.
Title : , Chemosphere, 358:142156 (2024)
Original Paper
Escherichia coli removal in down-flow hanging sponge reactors: insights from laboratory reactor studies

Presenter : Tomioka N., Tran P.T., Aoki M., Takemura Y., Syutsubo K.
Title : , Journal of Environmental Science and Health, Part A, 59(6):295-304 (2024)
Original Paper
Behavior of Aeromonas, Arcobacter, and Mycobacterium in freshwater microcosms polluted with domestic wastewater

Presenter : Aoki M., Nguyen Pham H.D., Takemura Y., Tomioka N., Ebie Y., Syutsubo K.
Title : , Water, Air, & Soil Pollution, 235:771 (2024)
Original Paper
Effect of long-term ammonia deficiency on nitrification performance and microbial dynamics in downflow sponge biofilm reactor

Presenter : Loi J.X., Syutsubo K., Rabuni M.F., Takemura Y., Aoki M., Chua A.S.M.
Title : , Journal of Cleaner Production, 481:144168 (2024)
Original Paper
Application of an anaerobic reactor for the treatment of sulfide-rich wastewater using biogas for H2S removal

Presenter : Onodera T., Takemura Y., Aoki M., Syutsubo K.
Title : , Water Science and Technology, 90(11):3029-3040 (2024)
Original Paper
Isolation and physiological properties of methanogenic archaea that degrade tetramethylammonium hydroxide

Presenter : Iguchi A., Takemura Y., Danshita T., Kurihara T., Aoki M., Hori S., Shigematsu T., Syutsubo K.
Title : , Applied Microbiology and Biotechnology, 107:3047-3056 (2023)
Original Paper
Enhanced sulfide removal by gas stripping in a novel reactor for anaerobic wastewater treatment

Presenter : Onodera T., Takemura Y., Aoki M., Syutsubo K.
Title : , Water Science & Technology, 87(9):2223 (2023)
Original Paper
A quantitative sequencing method using synthetic internal standards including functional and phylogenetic marker genes

Presenter : Koike K., Honda R., Aoki M., Yamamoto-Ikemoto R., Syutsubo K., Matsuura N.
Title : , Environmental Microbiology Reports, 15(6):497-511 (2023)
Original Paper
Quantitative detection and reduction of potentially pathogenic bacterial groups of Aeromonas, Arcobacter, Klebsiella pneumoniae species complex, and Mycobacterium in wastewater treatment facilities

Presenter : Aoki M., Takemura Y., Kawakami S., Yoochatchaval W., Tran P. T., Tomioka N., Ebie Y., Syutsubo K.
Title : , PLoS ONE, 18(9):e0291742 (2023)
Original Paper
Effect of inoculum sources on autotrophic nitrogen removal in anaerobic hollow fiber membrane reactors

Presenter : Tran P. T., Hatamoto M., Aoki M., Watari T., Syutsubo K., Yamaguchi T.
Title : , Environmental Technology & Innovation, 26:102375 (2022)
Original Paper
Sulfate differentially stimulates but is not respired by diverse anaerobic methanotrophic archaea

Presenter : Yu H., Skennerton C.T., Chadwick G.L., Leu A.O., Aoki M., Tyson G.W., Orphan V.J.
Title : , The ISME Journal, 16:168-177 (2022)
Original Paper
Hexavalent Chromium Removal and Prokaryotic Community Analysis in Glass Column Reactor Packed with Aspen Wood as Solid Organic Substrate

Presenter : Aoki M., Okubo K., Kusuoka R., Watari T., Syutsubo K., Yamaguchi T.
Title : , Applied Biochemistry and Biotechnology, 194(4):1425-1441 (2022)
Original Paper
Effect of formic acid inflow on microbial properties of the anaerobic granular sludge in a UASB reactor

Presenter : Syutsubo K., Miyaoka Y., Danshita T., Takemura Y., Aoki M., Tomioka N., Sumino H., Watari T., Yamaguchi T.
Title : , Journal of Environmental Science and Health, Part A, 57(5):413-419 (2022)
Original Paper
Manganese oxidation and prokaryotic community analysis in a polycaprolactone-packed aerated biofilm reactor operated under seawater conditions

Presenter : Aoki M., Miyashita Y., Miwa T., Watari T., Yamaguchi T., Syutsubo K., Hayashi K.
Title : , 3 Biotech, 12(9):187 (2022)
Original Paper
Effects of sulfate concentration on anaerobic treatment of wastewater containing monoethanolamine using an up-flow anaerobic sludge blanket reactor

Presenter : Takemura Y., Aoki M., Danshita T., Iguchi A., Ikeda S., Miyaoka Y., Sumino H., Syutsubo K.
Title : , Journal of Hazardous Materials, 440:129764 (2022)
Original Paper
Metagenome-Assembled Genome Sequence of Marine Rhizobiaceae sp. Strain MnEN-MB40S, Obtained from Manganese-Oxidizing Enrichment Culture

Presenter : Aoki M., Nakahara N., Kusube M., Syutsubo K.
Title : , Microbiology Resource Announcements, 11(9):e00645-22 (2022)
Original Paper
A pilot-scale study of a down-flow hanging sponge reactor as a post-treatment for domestic wastewater treatment system at short hydraulic retention times

Presenter : Takemura Y., Yoochatchaval W., Danshita T., Miyaoka Y., Aoki M., Tran P.T., Tomioka N., Ebie Y., Syutsubo K.
Title : , Journal of Water Process Engineering, 50:103313 (2022)
Original Paper
Anaerobic reactor with a phase separator for enhancing sulfide removal from wastewater by gas stripping

Presenter : Onodera T., Takemura Y., Aoki M., Syutsubo K.
Title : , Bioresource Technology Reports, 20(101216): (2022)
Original Paper
Sediment Microbiota in Response to Circuitry of Sediment Microbial Fuel Cells, Revealed by 16S rRNA Gene Amplicon Sequencing

Presenter : Takemura Y., Aoki M., Tran P.T., Tomioka N., Kubota K., Matsuura N., Syutsubo K.
Title : , Microbiology Resource Announcements, 10(46):e00984-21 (2021)

View all

A biofilm reactor packed with aspen wood and polyurethane sponge for biological manganese(II) oxidation and minor metal recovery

Presenter : Aoki M., Hayashi K., Wakui N., Hirakata Y., Watari T., Yamaguchi T., Syutsubo K.
Title : , World Journal of Microbiology and Biotechnology, 41(12):491 (2025)
Downflow sponge biofilm reactors for polluted raw water treatment: Performance optimisation, kinetics, and microbial community

Presenter : Loi J.X., Syutsubo K., Rabuni M.F., Takemura Y., Aoki M., Chua A.S.M.
Title : , Chemosphere, 358:142156 (2024)
Escherichia coli removal in down-flow hanging sponge reactors: insights from laboratory reactor studies

Presenter : Tomioka N., Tran P.T., Aoki M., Takemura Y., Syutsubo K.
Title : , Journal of Environmental Science and Health, Part A, 59(6):295-304 (2024)
Behavior of Aeromonas, Arcobacter, and Mycobacterium in freshwater microcosms polluted with domestic wastewater

Presenter : Aoki M., Nguyen Pham H.D., Takemura Y., Tomioka N., Ebie Y., Syutsubo K.
Title : , Water, Air, & Soil Pollution, 235:771 (2024)
Effect of long-term ammonia deficiency on nitrification performance and microbial dynamics in downflow sponge biofilm reactor

Presenter : Loi J.X., Syutsubo K., Rabuni M.F., Takemura Y., Aoki M., Chua A.S.M.
Title : , Journal of Cleaner Production, 481:144168 (2024)
Application of an anaerobic reactor for the treatment of sulfide-rich wastewater using biogas for H2S removal

Presenter : Onodera T., Takemura Y., Aoki M., Syutsubo K.
Title : , Water Science and Technology, 90(11):3029-3040 (2024)
Isolation and physiological properties of methanogenic archaea that degrade tetramethylammonium hydroxide

Presenter : Iguchi A., Takemura Y., Danshita T., Kurihara T., Aoki M., Hori S., Shigematsu T., Syutsubo K.
Title : , Applied Microbiology and Biotechnology, 107:3047-3056 (2023)
Enhanced sulfide removal by gas stripping in a novel reactor for anaerobic wastewater treatment

Presenter : Onodera T., Takemura Y., Aoki M., Syutsubo K.
Title : , Water Science & Technology, 87(9):2223 (2023)
A quantitative sequencing method using synthetic internal standards including functional and phylogenetic marker genes

Presenter : Koike K., Honda R., Aoki M., Yamamoto-Ikemoto R., Syutsubo K., Matsuura N.
Title : , Environmental Microbiology Reports, 15(6):497-511 (2023)
Quantitative detection and reduction of potentially pathogenic bacterial groups of Aeromonas, Arcobacter, Klebsiella pneumoniae species complex, and Mycobacterium in wastewater treatment facilities

Presenter : Aoki M., Takemura Y., Kawakami S., Yoochatchaval W., Tran P. T., Tomioka N., Ebie Y., Syutsubo K.
Title : , PLoS ONE, 18(9):e0291742 (2023)
Effect of inoculum sources on autotrophic nitrogen removal in anaerobic hollow fiber membrane reactors

Presenter : Tran P. T., Hatamoto M., Aoki M., Watari T., Syutsubo K., Yamaguchi T.
Title : , Environmental Technology & Innovation, 26:102375 (2022)
Sulfate differentially stimulates but is not respired by diverse anaerobic methanotrophic archaea

Presenter : Yu H., Skennerton C.T., Chadwick G.L., Leu A.O., Aoki M., Tyson G.W., Orphan V.J.
Title : , The ISME Journal, 16:168-177 (2022)
Hexavalent Chromium Removal and Prokaryotic Community Analysis in Glass Column Reactor Packed with Aspen Wood as Solid Organic Substrate

Presenter : Aoki M., Okubo K., Kusuoka R., Watari T., Syutsubo K., Yamaguchi T.
Title : , Applied Biochemistry and Biotechnology, 194(4):1425-1441 (2022)
Effect of formic acid inflow on microbial properties of the anaerobic granular sludge in a UASB reactor

Presenter : Syutsubo K., Miyaoka Y., Danshita T., Takemura Y., Aoki M., Tomioka N., Sumino H., Watari T., Yamaguchi T.
Title : , Journal of Environmental Science and Health, Part A, 57(5):413-419 (2022)
Manganese oxidation and prokaryotic community analysis in a polycaprolactone-packed aerated biofilm reactor operated under seawater conditions

Presenter : Aoki M., Miyashita Y., Miwa T., Watari T., Yamaguchi T., Syutsubo K., Hayashi K.
Title : , 3 Biotech, 12(9):187 (2022)
Effects of sulfate concentration on anaerobic treatment of wastewater containing monoethanolamine using an up-flow anaerobic sludge blanket reactor

Presenter : Takemura Y., Aoki M., Danshita T., Iguchi A., Ikeda S., Miyaoka Y., Sumino H., Syutsubo K.
Title : , Journal of Hazardous Materials, 440:129764 (2022)
Metagenome-Assembled Genome Sequence of Marine Rhizobiaceae sp. Strain MnEN-MB40S, Obtained from Manganese-Oxidizing Enrichment Culture

Presenter : Aoki M., Nakahara N., Kusube M., Syutsubo K.
Title : , Microbiology Resource Announcements, 11(9):e00645-22 (2022)
A pilot-scale study of a down-flow hanging sponge reactor as a post-treatment for domestic wastewater treatment system at short hydraulic retention times

Presenter : Takemura Y., Yoochatchaval W., Danshita T., Miyaoka Y., Aoki M., Tran P.T., Tomioka N., Ebie Y., Syutsubo K.
Title : , Journal of Water Process Engineering, 50:103313 (2022)
Anaerobic reactor with a phase separator for enhancing sulfide removal from wastewater by gas stripping

Presenter : Onodera T., Takemura Y., Aoki M., Syutsubo K.
Title : , Bioresource Technology Reports, 20(101216): (2022)
Sediment Microbiota in Response to Circuitry of Sediment Microbial Fuel Cells, Revealed by 16S rRNA Gene Amplicon Sequencing

Presenter : Takemura Y., Aoki M., Tran P.T., Tomioka N., Kubota K., Matsuura N., Syutsubo K.
Title : , Microbiology Resource Announcements, 10(46):e00984-21 (2021)

View all

Research Result (Presentation)

all
Research Presentation

Research Presentation
Isolation and Characterization of IPA- and Acetone-Degrading Microorganisms from UASB Granular Sludge

Presenter : Iguchi A., Orui M., Hori S., Shigematsu T., Nguyen Pham H.D., Tomioka N., Aoki M., Syutsubo K.
Name of Society : The Water and Environment Technology Conference 2025 (WET2025) (2025)
Name of Proceedings : PROGRAM AND ABSTRACTS, 54
Research Presentation
Microorganism distribution and their in situ activities in anaerobic granular sludge with acetone as the main substrate

Presenter : Nguyen Pham H.D., Aoki M., Iguchi A., Watari T., Hatamoto M., Yamaguchi T., Syutsubo K.
Name of Society : 15th Asian Symposium on Microbial Ecology (ASME2025) (2025)
Name of Proceedings : Abstracts, 101
Research Presentation
Development of an anaerobic reactor removing sulfides using biogas produced in the reactor

Presenter : Onodera T., Takemura Y., Aoki M., Syutsubo K.
Name of Society : 18th IWA World Conference on Anaerobic Digestion (2024)
Name of Proceedings : Conference Proceedings, 434-437
Research Presentation
Enhancement of anaerobic degradation of acetone in an UASB system by feeding 2-propanol

Presenter : Nguyen P.H.D., Syutsubo K., Aoki M., Iguchi A., Yamaguchi T.
Name of Society : The 14th International Symposium on Southeast Asian Water Environment (SEAWE-14) (2024)
Name of Proceedings : Abstracts
Research Presentation
Effect of long-term ammonia deficiency on nitrification performance and microbial dynamics in Downflow Sponge Biofilm (DSB) reactor

Presenter : Loi J.X., Aoki M., Rabuni M.F., Takemura Y., Syutsubo K., Chua A.S.M.
Name of Society : The 14th International Symposium on Southeast Asian Water Environment (SEAWE-14) (2024)
Name of Proceedings : abstracts
Research Presentation
Potential evaluation of autotrophic denitrification in sediment bio-electrochemical systems

Presenter : Tran P., Takemura Y., Aoki M., Tomioka N., Syutsubo K.
Name of Society : 2023 Asia-Pacific International Society of Microbial Electrochemistry and Technology (AP-ISMET) Conference (2023)
Name of Proceedings : Abstracts
Research Presentation
Genomic and Transcriptomic Analysis of a Novel Methanogenic Archaeon, Strain NY-STAYD for TMAH Degradation

Presenter : Iguchi A., Yamaguchi T., Kayamori R., Takemura Y., Aoki M., Hori S., Shigematsu T., Syutsubo K.
Name of Society : Water and Environment Technology Conference Online 2023 (2023)
Name of Proceedings : PROGRAM and ABSTRACTS, 28
Research Presentation
Effects of sulfate concentration on anaerobic treatment of monoethanolamine containing wastewater by UASB under psychrophilic conditions

Presenter : Takemura Y., Syutsubo K., Aoki M., Danshita T., Iguchi A.
Name of Society : The 2021 International Conference on the "Challenges in Environmental Science and Engineering" (CESE-2021) (2021)
Name of Proceedings : CESE-2021 ABSTRACTS BOOK, 46
Research Presentation
Effect of inflow of formic acid on microbial properties of anaerobic granular sludge in UASB reactor

Presenter : Syutsubo K., Miyaoka Y., Danshita T., Takemura Y., Aoki M.
Name of Society : The 2021 International Conference on the "Challenges in Environmental Science and Engineering" (CESE-2021) (2021)
Name of Proceedings : CESE-2021 ABSTRACTS BOOK, 32

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Development of an anaerobic reactor removing sulfides using biogas produced in the reactor

Presenter : Onodera T., Takemura Y., Aoki M., Syutsubo K.
Name of Society : 18th IWA World Conference on Anaerobic Digestion (2024)
Name of Proceedings : Conference Proceedings, 434-437
Enhancement of anaerobic degradation of acetone in an UASB system by feeding 2-propanol

Presenter : Nguyen P.H.D., Syutsubo K., Aoki M., Iguchi A., Yamaguchi T.
Name of Society : The 14th International Symposium on Southeast Asian Water Environment (SEAWE-14) (2024)
Name of Proceedings : Abstracts
Effect of long-term ammonia deficiency on nitrification performance and microbial dynamics in Downflow Sponge Biofilm (DSB) reactor

Presenter : Loi J.X., Aoki M., Rabuni M.F., Takemura Y., Syutsubo K., Chua A.S.M.
Name of Society : The 14th International Symposium on Southeast Asian Water Environment (SEAWE-14) (2024)
Name of Proceedings : abstracts
Genomic and Transcriptomic Analysis of a Novel Methanogenic Archaeon, Strain NY-STAYD for TMAH Degradation

Presenter : Iguchi A., Yamaguchi T., Kayamori R., Takemura Y., Aoki M., Hori S., Shigematsu T., Syutsubo K.
Name of Society : Water and Environment Technology Conference Online 2023 (2023)
Name of Proceedings : PROGRAM and ABSTRACTS, 28
Effects of sulfate concentration on anaerobic treatment of monoethanolamine containing wastewater by UASB under psychrophilic conditions

Presenter : Takemura Y., Syutsubo K., Aoki M., Danshita T., Iguchi A.
Name of Society : The 2021 International Conference on the "Challenges in Environmental Science and Engineering" (CESE-2021) (2021)
Name of Proceedings : CESE-2021 ABSTRACTS BOOK, 46
Effect of inflow of formic acid on microbial properties of anaerobic granular sludge in UASB reactor

Presenter : Syutsubo K., Miyaoka Y., Danshita T., Takemura Y., Aoki M.
Name of Society : The 2021 International Conference on the "Challenges in Environmental Science and Engineering" (CESE-2021) (2021)
Name of Proceedings : CESE-2021 ABSTRACTS BOOK, 32
Isolation and Characterization of IPA- and Acetone-Degrading Microorganisms from UASB Granular Sludge

Presenter : Iguchi A., Orui M., Hori S., Shigematsu T., Nguyen Pham H.D., Tomioka N., Aoki M., Syutsubo K.
Name of Society : The Water and Environment Technology Conference 2025 (WET2025) (2025)
Name of Proceedings : PROGRAM AND ABSTRACTS, 54
Microorganism distribution and their in situ activities in anaerobic granular sludge with acetone as the main substrate

Presenter : Nguyen Pham H.D., Aoki M., Iguchi A., Watari T., Hatamoto M., Yamaguchi T., Syutsubo K.
Name of Society : 15th Asian Symposium on Microbial Ecology (ASME2025) (2025)
Name of Proceedings : Abstracts, 101
Potential evaluation of autotrophic denitrification in sediment bio-electrochemical systems

Presenter : Tran P., Takemura Y., Aoki M., Tomioka N., Syutsubo K.
Name of Society : 2023 Asia-Pacific International Society of Microbial Electrochemistry and Technology (AP-ISMET) Conference (2023)
Name of Proceedings : Abstracts

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Aoki Masataka

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