Asplanchna
- Asplanchna girodi
- Asplanchna sieboldi
[ref. ID; 754]
Test system
Strains
Asplanchna girodi was collected 19 July, 1977 in Golf Course Pond. Three malic enzyme genotypes S, H, and F. These rotifers were cultured in Pourriot-Gilbert (P-G) medium (Gilbert 1963) and fed Paramecium tetraurelia. The paramecia were cultured on Enterobacter aerogenes grown in Cerophyl medium (Sonnenborn 1950) at 28 degrees C.
Toxicants
The blue-greens Anabaena flos-aquae (Strain F-1) and Lyngbya sp. (Strain F-2) were isolated from Golf Course Pond near Tampa, Florida in July 1977.
Test design
A single mature female in 1 ml P-G medium with 2,300+/-100 paramecia in a 13x100 mm test tube.
- Control paramecia only, 40 tubes - paramecia +
- 0.004 mg Anabaena dry weight/ml, 40 tubes - paramecia +
- 0.010 mg Anabaena dry weight/ml, 40 tubes - paramecia +
- 0.020 mg Anabaena dry weight/ml, 40 tubes - paramecia +
- 0.035 mg Anabaena dry weight/ml, 40 tubes - paramecia +
- 0.350 mg Anabaena dry weight/ml, 40 tubes - paramecia +
- 0.004 mg Lyngbya sp. dry weight/ml, 40 tubes - paramecia +
- 0.010 mg Lyngbya sp. dry weight/ml, 40 tubes - paramecia +
- 0.020 mg Lyngbya sp. dry weight/ml, 40 tubes - paramecia +
- 0.035 mg Lyngbya sp. dry weight/ml, 40 tubes - paramecia +
- 0.350 mg Lyngbya sp. dry weight/ml, 40 tubes - paramecia +
Tubes were placed on a shaker at 175 rpm in constant light (5,000 lux) at 28 degrees C for 72 hr.
Measurements
Number of female.
Evaluations
Reproductive rate.
[ref. ID; 3329]
Test system
Over 120 days sublethal toxicity (behavioral toxicity, reproductive toxicity, and both behavioral and reproductive toxicity)
Toxicants
Pentachlorophenol (PCP).
Test design/concentrations
6 prey species (Brachionus calyciflorus, Keratella cochlearis, Philodina acuticornis, Lepadella patella, Trichocerca pusilla, and Plationus patulus) x 4 concentrations (0, 110, 190 and 330 mg/l).
Evaluations
Population growth rate (Gr); maximum population densities (Dmax); the percent of carrying capacity at Dmax (%K).
[ref. ID; 1973]
Test system
24 hr acute toxicity test (B. calyciflous (prey) and Asplanchna sieboldi (predator) interaction)
Strains
Originally isolated from Lake Chapultepec in Mexico city.
Toxicants
Methyl parathion.
Temperature & Light condition
25 degrees C. Continuous diffused fluorescent illumination.
Test design
25-ml capacity transparent vials containing 20 ml EPA medium. B. calyciflorus (food: Chlorella vulgaris (0.25x10E6 cells/ml)) treated in four ways as prey for Asplanchna viz.
- 1) control - prey offered in living conditions;
- 2) prey living but sublethally exposed to methyl parathion (10 mg/L for 2 hours);
- 3) cold-killed prey (froze at 0 degrees C for 24 hr);
- 4) toxicant killed prey (100 mg/L for 2 hours).
For food concentrations viz. 2.5, 5.0, 10.0 and 20.0 ind./ml, 5 replicates (4 food types x 4 food densities x 5 replicates).
Measurements/observeations
Population growth rate.
Evaluations
LC50.
[ref. ID; 6088]
Test system
18-hr acute toxicity
Strains
From Professor G.W. Salt of the University of Califoria at Davis, asexual (amictic condition).
Toxicants
Ten crude oils (Norman Wells, Sweet Mixed Blend, Murban, Prudhoe Bay, Sour Blend Alberta, Bow River, Lloyd Minster, Lagomedio, Alberta, La Rosa).
Temperature
22+/-1 degrees C.
Test design
- Run 1: Food Paramecium tetraurelia (from Professor G.W. Salt) - Enterobacter aerogenes
Covered solid watchglasses containing 1 ml of SGM (0.1% (w/v) Scottish Grass Medium) + Crude oil (5-200 ul) + Approx. 100 cells of P. tetraurelia, 9 replicates, gently shaken (100 strokes min-1)
- Run 2: Food Colpidium colpoda
Covered solid watchglasses containing 1 ml of SGM + Colpidium (100 cells)
Covered solid watchglasses containing 1 ml of SGM + Colpidium (100 cells) + Norman Wells crude oil (50 ul)
Covered solid watchglasses containing 1 ml of SGM + Colpidium (100 cells) + partially degraded Norman Wells Oil (by subjecting 2% (V/V) crude oil in 0.1% SGM to the degradative action of the oil-utilising bacterium, Acinetobacter H01-N, for 30 days).
Covered solid watchglasses containing 1 ml of SGM + Colpidium (by harvesting ciliates reared in bacterised batch culture with partially degraded Norman Wells oil for at least 20 days, 100 cells).
Measurements
- Run 1: Suvival number.
- Run 2: Consumption rates of C. colpoda.
Endpoints
Mortility, longevity, and total neonate production per generation.