Medium composition
- Water
- Soil
1. Water
1.1. APW medium
Medium composition
0.5 mM NaCl, 0.2 mM NaHCO3, 0.05 mM KCl, 0.4 mM CaCl2.
Purpose for use
Artificial pond water.
1.2. ASPM medium (Guillard, 1983)
Medium composition
- 7ppt salinity: NaCl 5.28 g, KCl 0.17 g, CaCl2 0.25 g, MgCl2/6H2O 0.92 g, MgSO4/7H2O 1.12 g, NaHCO3 0.08 g, in 1 L of NANOpure deionized water, pH 7.5.
- 15ppt salinity: NaCl 11.31 g, KCl 0.36 g, CaCl2 0.54 g, MgCl2/6H2O 1.97 g, MgSO4/7H2O 2.39 g, NaHCO3 0.17 g, in 1 L of NANOpure deionized water, pH 8.0.
Purpose for use
Synthetic seawater.
1.3. Chalkley's medium
Medium composition
0.01% (w/v) sodium chloride, 0.0004% (w/v) potassium chloride, 0.0006% (w/v) calcium chloride.
Purpose for use
Isotonic inorganic salt solution.
1.4. EPA medium (EPA, 1985)
Medium composition
NaHCO3 96 mg, CaSO4/2H2O (or CaSO4) 60 mg, MgSO4 60 mg, KCl 4 mg in deionized water 1L. Deionized base water was obtained from a Barnstead NANOpure II system with one pretreatment, one high-capacity, and two ultrapure cartridges. pH 7.5.
Purpose for use
Synthetic freshwater.
2.1. ASTM artificial soil (ASTM, 1997)
Soil composition
10% sphagnum peat moss, 20% kaolin clay, 70% sand (grade 70. particle size 0.1-0.3 mm).
The ASTM soil is of the same composition as the OECD soil (OECD 1984) and serves as a standard test medium. (ref. ID; 3301)
2.2. OECD artificial soil (OECD Guideline No.207) (OECD, 1984)
Soil composition
- 1. 10% <0.5 mm ground sphagnum peat, 20% kaolin clay, 69% fine sand, 1% calcium carbonate (by dry weight).
- 2. 70% sand, 20% kaolin clay, 10% organic matter (as Sphagnum peat) (by dry weight), pH 6.0+/-0.5 (prepared by powdered calcium carbonate).