Holosticha kessleri
[ref. ID; 6111]
Test system
Acute toxicity (24-hr LC50)
Strains
The organisms was obtained, initially, from Stirone Stream (northern Italy), a freshwater environment free from known toxicants and, in particular, from heavy metals. The culture medium was constituted of boiled rice and wheat grain in 10 ml of filtered natural water. 20+/-1 degrees C, photoperiod of 16:8 hr light:dark.
Toxicant/concentrations
NiCl2/6H2O (0.99, 1.12, 1.25, 1.37 ppm).
Test design
Tissue culture plates with 24 wells, 3 replicates.
Measurements
Mortality or the survivorship.
Evaluations
LC50 using the probit method.