Blepharisma
- Blepharisma americanum
- Blepharisma undulans
[ref. ID; 6011]
Test system
Acute toxicity (24-hr LC50)
Strains
From the aeration tank of activated sludge works designated for the treatment of domestic wastes in the district of Reggio Emilia, northern Italy.
Toxicants
Hydrated cadmium chloride, hydrated copper chloride, zinc chloride, mercury chloride.
Test design
Costar tissue culture plates with 24 wells were employed. Two replicates of 12 organisms each were run of each test concentration.
Evaluations
LC50 using the probit method.
[ref. ID; 496]
Test system
Acute toxicity (9-hr LC50 and 9-hr LC10)
Strains
Axenic culture.
Toxicants
Chlorex (sodium chlorate) 55, MCPA 750, Dipror (Dichlorprop) 640, Matrigon (chlopyralide), Benlate, Sumicidin (fenvalerate).
Test design/concentrations
In tubes with amoeba saline ("Modified Neff's Amoeba Saline" (Page 1967): NaCl 0.120, MgSO4/7H2O 0.004, CaCl2/2H2O 0.004, Na2HPO4 0.142, KH2PO4 0.136 g litre-1), bacteria (Enterobacter aerogenes), and 15 ml of the ciliate suspension.
Measurements
MPN method and Absorbance method.
Evaluations
LC10 and LC50.