Heterocephalobus pauciannulatus
[ref. ID; 7025]
Test system
Phenotypic plasticity vs. concentration-response relationships
Strains
Toxicants
CuCl2/2H2O
Test design
The water used for culturing and experiments contained a defined mixture of minerals resembling those found in intestitial water of sandy forest soil. pH 6.0+/-0.1.
Life-cycle studies were conducted in agar in multicompartment plates (Greiner 662160). Agar droplets (70 ul, 0.5%) were applied on the inside of the lid. Acinetobacter johnsonion (2x10E8 cells/ml). Each compartment of the bottom plate contained 1 ml water, and plates were sealed with Parafilm to prevent evaporation. 0, 12.4, 22.4, 40.4, 72.8, and 131 uM copper in agar.
Eggs were individually transffered to the agar droplets. 24 replicates per each concentration.
Measurements/observations
Juvenile survival and period. Daily reproduction and reproductive period. Fitness.
Evaluations
EC20 using nonlinear regression procedures as described in Bruce and Versteeg.