Ref ID : 948
Cees A.M. Broers, Claudius K. Stumm, and Godfried D. Vogels; Axenic cultivation of the anaerobic free-living ciliate Trimyema compressum. J.Protozool. 38(5):507-511, 1991
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The strains N of Trimyema compressum an anaerobic free-living ciliate, was cultivated axenically in a medium containing a buffered salt solution, yeast extract, trypticase, and glutathione. Dead bacteria were indispensable as food; a culture of the ciliate together with heat-killed Klebsiella pneumoniae has been established for more than one year. In the medium described, the ciliates grow to higher cell density than in cultures with living bacteria as food. During the process of axenization, a nonmethanogenic bacterial endosymbiont was lost. In the microbodies of T. compressum, hydrogenase could be localized by the technique of indirect immuno-fluorescence.