Ref ID : 93
Williams, N.E., Honts, J.E., and Stuart, K.R.; Properties of microtubule-free cortical residues isolated from Paramecium tetraurelia. J.Cell Sci. 92:427-432, 1989
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Notes
We have found that shape-preserving residues devoid of microtubules can be prepared from Paramecium using Triton X-100 at high ionic strength. These residues contain many proteins, including one showing antigenic relationship to chicken alpha-spectrin, and three showing antigenic relationship to Tetrahymena cortical proteins. These antigens have been localized by immunofluorescence microscopy, and the isolated cortical residues have been characterized ultrastructurally. These preparations should be useful in detailed studies of the structure, development and evolutionary relationships of cortical architecture in ciliated protozoa.