Ref ID : 900
Cicily Chapman-Andresen; Studies on pinocytosis in amoebae. Compt.Rend.Trav.Lab.Carlsberg 33(3):73-244, 1970
Reprint
In File
Notes
The intensity of pinocytosis in amoebae is estimated by the use of two methods, a semi-quantitative and a quantitative, respectively, and the errors involved in the methods are discussed. The morphology of pinocytosis in sodium chloride and in other inducing solutions is described for different species of amoebae with regard to channel formation and appearance of pinocytosis vacuoles. The influence on the intensity of pinocytosis of different factors such as pH, temperature, the presence of buffers in the inducing solutions, enucleation and the physiological state of the amoebae is investigated. In addition to inorganic salts, amino acids, some proteins and biological strains are found to induce pinocytosis, and the interaction between some of these inducers and some non-inducers is discussed. The mucopolysaccharides on the outer surface of the plasmalemma play an important role in pinocytosis, and the first phase of the process is considered as an interaction between the mucus and the inducer. It was found that different inducers show different degrees of binding to the mucus, and it is suggested that alterations in the state of the mucocus coat may influence the interactions of the different solutions investigated. A comparison is made between pinocytosis vacuoles and other inclusion bodies and enzymes in the cell is discussed.