Ref ID : 499
T.A. Forge, M.L. Berrow, J.F. Darbyshire, and A. Warren; Protozoan bioassays of soil amended with sewage sludge and heavy metals, using the common soil ciliate Colpoda steinii. Biol.Fertil.Soils 16:282-286, 1993
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The common soil protozoan Colpoda steinii used to study the toxicity of sulphate solutions of Ni, Cd, Cu, and Zn. The growth of C. steinii was reduced by 50% in the presence of 0.10, 0.22, 0.25, and 0.85 mg/l of Ni, Cd, Cu and Zn, respectively, during 24 hr of incubation at 25 degrees C, as calculated from a regression analysis of probit-transformed data. The same growth assay was used to assess the toxicity of soil solutions extracted by centrifugation from soil samples of field plots of a grass/clover ley on a sandy loam treated with sewage sludge spiked with additional Cd, Cu, Cr, Ni, Pb, Zn at concentrations either equivalent to or twice the limits for heavy metals recommended in recent EC guidelines (Commission of European Communities directive 86/278/EEC). The toxicity of these soil solutions varied with the season of the year. None of the soil solutions extracted in winter (February 1991) inhibited the growth of C. steinii. In summer (July 1991), the growth was reduced in solutions extracted from plots that were amended with sludge plus additional Zn or Ni at twice the maxima recommended by the EC. The changes in toxicity to C. steinii of the soil solutions between February and July were positively correlated with increases in heavy metal concentrations of Zn and Ni between winter and summer. These preliminary results suggest that regular protozoan bioassays may be used to monitor the biological availability of heavy metals in soils, especially when combined with other microbial assays and with chemical analysis of soil solutions.