Ref ID : 419
Sawada, M. and Enesco, H.E.; A study of dietary restriction and lifespan in the rotifer Asplanchna brightwelli monitored by chronic neutral red exposure. Exp.Gerontol. 19:329-334, 1984
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The rotifer Asplanchna brightwelli could be exposed to concentrations of neutral red at 0.1 to 0.75 microgram/ml for two consecutive generations with no adverse effect on the lifespan or fecundity of either generation. Chronic exposure to higher concentrations of neutral red caused reductions in both lifespan and fecundity of the rotifers. Because neutral red stains the Paramecium which serve as a food source for the rotifers, the extent of red coloration in each rotifer's gut gave an approximation of how much food that rotifer had consumed. The Paramecium concentration had to be reduced to 100 per ml before pale gut coloration provided clear evidence of reduced food intake or dietary restriction. The group of rotifers with reduced food intake had a significantly longer lifespan than any other group. Their lifespan was 14.2% longer than that of control rotifers.