Ref ID : 134
Wolfgang Eichler; Regulation of Ornithine Decarboxylase Activity in the Growth Cycle of Tetrahymena thermophila. J.Protozool. 37(4):273-277, 1990
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Cells of the ciliated protozoan Tetrahymena thermophila, grown in proteose peptone medium up to late logarithmic phase, harvested by centrifugation, and resuspended in fresh medium to almost the same cell density, underwent one more division cycle within 5 hour after inoculation, thereafter being definitely in full stationary phase. This growth cycle proved to be a useful tool to investigate the activation and deactivation of ornithine decarboxylase ODC1 in Tetrahymena: In late logarithmic phase the cells contained a very low specific activity of ODC of about 3 nmol CO2.hr-1.mg-1 in the soluble protein fraction. After growth stimulation the activity was increased up to 100-fold within 1 hour. This high activity was maintained for about 5 hour-about as long as division activity-then rapidly declined with a half life time (t(1/2)) of about 15 min to the original low level. Inhibition assays with cycloheximide and actinomycin D revealed that: i. the rapid increase of ODC activity was biphasic with one component of translation of preexisting mRNA and one component of translation of newly transcribed mRNA; ii. the t(1/2) of the mRNA of ODC was estimated to be about 2 hour; iii. inhibition of protein biosynthesis before ODC inactivation at 5 hour caused a decrease of ODC with a t(1/2) of 55 min instead of 15 min. These findings suggest that ODC activity in Tetrahymena is regulated on both levels: transcription and translation and by an inactivating protein factor which is regulated at the level of biosynthesis.