Ref ID : 106
Esther (wajc)Lubzens, Rachel Fishler, and Viviane Berdugo-White; Induction of sexual reproduction and resting egg production in Brachionus plicatilis reared in sea water. Hydrobiologia 73:55-58, 1980
Reprint
In File
Notes
Brachionus plicatilis raised in our laboratory in sea water reproduces asexually even under high crowding conditions (at least 40 individuals per ml). Amictic females were induced to produce mictic females, males and resting eggs by reducing the concentration of the sea water culture medium. Mictic females and males appeared predominantly among the progeny produced by the amictic females during 4 days following their transfer into 25% sea water. Resting eggs appeared first 5-12 days after the onset of the experiment. Following the disappearance of males, the culture consisted of amictic females. Resting eggs produced by the method described above maybe presented for at least three months at -14 degrees C or by desiccation at room temperature. Under the appropriate experimental conditions, resting eggs hatch into amictic females. Since B. plicatilis is one of the most commonly used food souces of fish larvae in aquaculture, the methods reported here may offer stock to be used on demand.