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The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 7655

Pilar Delgado, Pilar Calvo, and E. Viscogliosi; Estimation of the Number of alpha-Tubulin Genes in Three Ciliated Protozoa. Arch.Protistenkd. 144:105-111, 1994

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In an attempt to study the multitubulin family in ciliated protozoa, we have estimated the number of alpha-tubulin genes present in the genome of three "Spirotrich" ciliates by using an alpha-tubulin probe from the ciliate Stylonychia lemnae. Southern hybridization analyses of single or double-digested DNA from ciliates Histriculus cavicola, Euplotes eurystomus and Stentor coeruleus revealed banding patterns consistent with the presence of three, two and at least nine alpha-tubulin genes in each species, respectively. Comparison of the hybridizing patterns of Histriculus and Euplotes digested DNAs showed a great similarity between both species. The existence of common restriction fragments is an evidence of a sequence homology at the genomic level and might attribute much weight to a close relation between both ciliates. The controversial relationships among "Spirotrich" ciliates are discussed.