Ref ID : 7621
Chennayil Nandakumar and Satendra Khera; Life Cycle and Reproduction in Female Pratylenchus mulchandi with Emphasis on the Development of Gonad. Zool.Anz.,Jena 193(3/4):287-296, 1974
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The life cycle and post-embryonic development of Pratylenchus mulchandi has been investigated by propagating the nematode on plant callus tissues. The length of the life cycle has been studied by inoculating single gravid females in agar plates containing pearl millet seedlings. Newly deposited eggs vary in development from the unicellular to multicellular stage. Only a single extra - vitellar polar body has been observed. Cleaved eggs in various stages of development even up to the first stage larva, are not uncommon in the body of the mother. There are four moults in the life cycle, the first occurring in the egg. The genital primordium of the second stage larva consists of four cells- two large germinal cells enclosed by two smaller somatic cells. The reproduction in this monosexual species seems to be parthenogenetic since no male specimens or sperm material have been encountered. The life cycle takes 24-36 days at room temperature (25-30 degrees C): 6-10 days for the egg to hatch, 15-20 days for the second stage larva to mature into adult and 3-6 days from maturation to egg laying.