Main Content

The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 7431

Adam Seegmiller and Glenn Herrick; A Short Internal Eliminated Sequence with Central Conserved Sequences Interrupting the LA-MSC Gene of the 81 Locus in the Hypotrichous Ciliates Oxytricha fallax and O. trifallax. J.Eukaryot.Microbiol. 45(1):55-58, 1998

Reprint

In File

Notes

IES-LA is a short Internal Eliminated Sequence interrupting LA-MSC, a protein-coding gene of the 81 locus of Oxytricha fallax and O. trifallax. IES-LA is precisely excised from the gene during development of the macronucleus. The internal eliminated sequence is bounded by CAAT...AATG, and thereby resembles a TBE1 transposon internal eliminated sequence insertion that is grossly shortened (4.1 kbp to 52-64 bp), consistent with the hypothesis that short IESs are degenerated ancient transposons. The pattern of sequence conservation between five alleles of IES-LA shows that it differs from previously characterized classes of ciliate short IESs: while many short IESs have conserved ends and diverged centers, IES-LA is more conserved in this center and its ends are diverged. This implies a excision mechanism for IES-LA that is distinct from those for other known Oxytricha IESs.