Ref ID : 7285
Donald E. Olins and Ada L. Olins; Inhibition of DNA Synthesis in the Macronuclear Replication Band of Euplotes eurystomus. J.Eukaryot.Microbiol. 40(4):459-467, 1993
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The replication band is a large, migrating macronuclear domain that is the site of DNA synthesis in hypotrichous ciliated protozoa. A number of agents that produce inactivation of this structure and its replicational activity are described here. These agents include heat shock, aphidicolin, cell crowding, various cAMP phosphodiesterase inhibitors and a calmodulin inhibitor. With the exception of aphidicolin, which has a direct inhibitory effect upon DNA polymerases, the mechanisms of inactivation are presently unknown. The inactivating properties of cAMP phosphodiesterase inhibitors suggest that intracellular cAMP levels may influence replication band structure and function.