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The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 7115

Chandni P. Kher, F. Paul Doerder, Jason Cooper, Pranvera Ikonomi, Undine Achilles-Day, Frithjof C. Kupper, and Denis H. Lynn; Barcoding Tetrahymena: Discriminating Species and Identifying Unknowns Using the Cytochrome c Oxidase Subunits I (cox-1) Barcode. Protist 162:2-13, 2011

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DNA barcording using the mitochondrial cytochrome c oxidase subunit (cox-1) gene has recently gained popularity as a tool for species identification of a variety of taxa. The primary objective of our research was to explore the efficacy of using cox-1 barcoding for species identification within the genus Tetrahymena. We first increased intraspecific sampling for Tetrahymena canadensis, and Tetrahymena hegewischi, Tetrahymena pyriformis, Tetrahymena rostrata, Tetrahymena thermophila, and Tetrahymena tropicalis. Increased sampling efforts show that intraspecific sequence divergence is typically less than 1%, though it may be more in some species. The barcoding also showed that some strains might be misidentified or mislabeled. We also used cox-1 barcodes to provide species identifications for 51 unidentified environmental isolates, with a success rate of 98%. Thus, cox-1 barcoding is an invaluable tool for protistologists, especially when used in conjunction with morphological studies.