Ref ID : 4823
J. Lukavsky and V. Cepak; DAPI Fluorescent Staining of DNA Material in Cyanelles of the Rhizopoda Paulinella chromatophora Lauterb. Arch.Protistenk 142:207-212, 1992
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Endocyanelles in the rhizopod Paulinella chromatophora Lauterb. were examined under a light microscope complemented by DAPI florescent staining. They resemble the cyanobacterium Synechococcus elongatus (Nag.) Nag. f. thermalis Geitl., of 14 examined species of the genus Synechococcus. They both have a similar size, the mode of cell division as well as the shape and structure of nucleoid. These findings confirm Pascher's (1929) suggestion that these endocyanelles belong to the genus Synechococcus Nag. The rhizopod contains 2-6 blue-green sausage-shaped endocyanelles (3-4 x 16-18 µm). They divide by invagination of the cell wall, upright to the long axis of the cyanelle, into almost equal parts. After DAPI staining, the central part of the cyanelles (nucleoids) emits intensive bluish fluorescence (as does the nucleus of the rhizopod). The fluorescence disappears after DNAse treatment, which proves the presence of DNA in DAPI-stained material in cyanelles.