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The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 4748

D.McL. Roberts and H. Causton; Silver Nitrate Impregnation of Ciliated Protozoa. Arch.Protistenk 135:299-318, 1988

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A modified silver nitrate impregnation procedure is described, based on the Chatton-Lwoff method. The major differences are (1) the post-fixation in da Fano's fixative is reduced to a single wash and (2) that the variability between preparations has been reduced by mixing da Fano's fixative with the gelatine mountant prior to mixing with the cells. The total time required for the new protocol is 2 hr from live culture to mounted slides.