Ref ID : 4680
Bernard Pelvat and Gerand De Haller; [Etude des proteines synthetisees au cours de la regeneration de la zone membranelles adorales chez Stentor coeruleus] (Study of the proteins synthesized during regeneration of the apical zone of membranelles in Stentor coeruleus). Arch.Protistenk 128:201-229, 1984
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Oral regeneration in Stentor coeruleus implicates reconstitution of an essential structure: the adoral zone of membranelles (AZM). Regeneration takes 8 hr. Action of protein inhibitors shows that regenerating cells are sensitive during the formation of the oral primordium. By Page and fluorography, we have analysed the proteins synthesized during regeneration in starved cells. It can be concluded that: - The use of starved cells allows one to avoid interference by syntheses during the cell cycle. - During regeneration, a large number of proteins are synthesized. - Reconstitution of the AZM is accomplished during the first 3-4 hr of regeneration (oral primordium), taking most of the tubulines from a preexisting cytoplasmic pool. - If the AZM is regenerated in the presence of H3-leucine, shed again and analysed by Page, tubulines are labelled (induction of tubulines synthesis) as are many other proteins. Thus, newly synthesized tubulines are used of regeneration in addition to those of the protein pool. - The pool of tubulines is restored during the last hours of regeneration. At this time the AZM is formed and migrates to its normal position. This last morphogenetic process is not dependant of protein synthesis since it is not sensitive to cycloheximide.