Ref ID : 4563
Angelika Preisfeld, Gabriele Scholten-Beck, and Hans Georg Ruppel; Cell Surface Glycoconjugates of Euglena gracilis (Euglenozoa): Modifications under Potassium and Magnesium Deficiency. Z.Naturforsch. 52c:33-41, 1997
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Biochemical and ultrastructural examinations on the pellicle of autotrophically grown Euglena gracilis were carried out after three days under potassium and magnesium deficiency. Cell-surface changes were detected by lectin assay. Compared to cells grown in complete medium, deficient cells become larger in shape, accompanied by rising carbohydrate, chlorophyll and protein content, bind more and other lectin molecules: an increase of mainly galactose and N-acetylgalatosamine receptors was observed. Investigations with the mucilage strains alcian blue and ruthenium red indicated that mucilaginous material is released under deficient conditions, whereas the control cells show a strong precipitate of these strains well inside the cells beneath the pellicle.