Main Content

The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 4547

A.A. Juknat, D. Dornemann, and H. Senger; Different Porphobilinogenases in Cytoplasm and Isolated Chloroplasts from Light-Grown Euglena gracilis Z. Z.Naturforsch. 44c:81-84, 1989

Reprint

In File

Notes

Lysed cells as well as different fractions of isolated, disrupted chloroplasts from light grown Euglena gracilis were tested for their capability of porphyrin biosynthesis. It is shown that the formation of the soluble porphobilinogenase (PBG-ase) fractions is inhibited by cycloheximide indicating its biosynthesis on 80S ribosomes. Whereas the formation of the membrane bound chloroplast PBG-ase is chloramphenicol sensitive. Different pH-optima are demonstrated for the two enzymes. From the presented results it is deduced that PBG-ase from chloroplasts is different from those isolated from dark grown cells.