Ref ID : 4321
Tae I. Ahn and Kwang W. Jeon; Antigenic Reactivity of a Monoclonal Antibody against Amoeba proteus Actin. J.Protozool. 36(6):560-562, 1989
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The reactivity of a monoclonal antibody against actin of Amoeba proteus with actins from other sources was examined. The monoclonal antibody cross-reacted with actins from vertebrate muscles, human erythrocytes, and Acanthamoeba castellanii, but it did not react with Naegleria gruberi actin. The amoeba actin was resolved into 3 bands with isoelectric points of 5.96, 6.03 and 6.10 in electrofocusing gels and they corresponded to 3 peptide spots reacting with the antibody on 2-dimensional immunoblots.