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The World of Protozoa, Rotifera, Nematoda and Oligochaeta

Ref ID : 4268

H.B. Kim and Kwang W. Jeon; A Monoclonal Antibody against a Symbiont-Synthesized Protein in the Cytosol of Symbiont-Dependent Amoebae. J.Protozool. 34(4):393-397, 1987

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A monoclonal antibody was obtained against a 29-kD polypeptide in the cytosol of a symbiont-bearing strain (xD) of Amoeba proteus and was used to determine the distribution of the antigen in amoebae. The 29-kD polypeptides (xD protein) are produced by bacterial endosymbionts that are necessary for the survival of host xD amoebae. Results of indirect immunofluorescent and electron-microscopic immunogold-labeling studies showed that the xD protein was present diffusely in the amoeba cytoplasm as well as in the symbiotic bacteria. The native protein containing 29-kD polypeptides was purified using an immunoaffinity column prepared with the monoclonal antibody and its molecular weight was determined to be 87,000.