Ref ID : 4230
B.L. Cable and D.T. John; Condition for Maximum Enflagellation in Naegleria fowleri. J.Protozool. 33(4):467-472, 1986
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Ameba to flagellate transformation in Nagleria fowleri (Lovell strain) was affected by growth temperature, phase of growth, strain of ameba, culture agitation, enflagellation temperature, enflagellation diluent, and cell concentration. Amebae transformed best when they were grown without agitation and enflagellated with agitation. Regardless of growth temperature (23, 30, 37, and 42 degrees C were tested), amebae transformed best at 37 degrees C. Enflagellation was greatest for cells harvested between 24 hr (mid-exponential) and 84 hr (late stationary) of growth.