Ref ID : 4229
K.K. Hjelm; Determinations of Volumes and Minimal Surface Areas of Individual Compressed Live Tetrahymena. J.Protozool. 33(4):454-456, 1986
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A method to detemine the volume of individual live Tetrahymena with improved precision has been developed. Cells are compressed between a microslide and coverslip and their volume calculated from their thickness and area determined by interference microscopy and from a photomicrograph, respectively. A formula has been derived to correct of the "edge error" which arises because the rounded edges of the cells are projected on the film as if they were rectangular. The minimal surface area of compressed cells, i.e. that necessary to surround the cells smoothly, can also be calculated. Cells may be recovered after measurements. The method may also be applied to other cells.