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Ref ID : 4222

Ignacio Bolivar and Jacqueline Guiard-Maffia; Expression of Surface Coat Glycoconjugates by Bacteria-Fed Tetrahymena. J.Protozool. 33(3):335-340, 1986

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We have shown that bacteria-fed Tetrahymena express at their surface and excrete into the medium a glycoconjugate absent in axenically grown cells. A preliminary analysis of the purified molecule is given. Immunolabeling of blotted surface extracts and fixed cells shows that bacteria-fed Tetrahymena build up a surface coat whose material originates totally or in part in the mucocysts. The glycoconjugate is located externally on the coat and mediates cell immobilization and immunolabeling by the serum. The results also indicate that axenic cells are probably devoid of surface coat.