Ref ID : 4112
T.I. Ahn and Kwang W. Jeon; Strain-Specific Proteins of Symbiont-Containing Amoeba proteus Detected by Two-Dimensional Gel Electrophoresis. J.Protozool. 30(4):713-715, 1983
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Two-dimensional gel electrophoresis was used to compare the protein composition of normal (D strain) and symbiont-containing (xD strain) A. proteus. Over 500 different peptide spots could be identified on a typical gel of cytosol proteins. In addition to differences in a few minor peptides, one prominent polypeptide with an isoelectric point of about 5.5 and a molecular weight of about 29,000 was present in the cytoplasm of symbiont-containing amoebae and in the endosymbionts but not in D-strain amoebae. When endosymbionts were removed from xD-strain amoebae, the xD-specific polypeptide also disappeared, suggesting that it is produced by the endosymbionts.