Ref ID : 4048
Anthony J. Luksas and J.A. Erwin; The Lipid Requirement of the Ciliated Protozoon Tetrahymena patula. J.Protozool. 30(1):8-13, 1983
Reprint
In File
Notes
The ciliated protozoon Tetrahymena patula can be grown in a chemically defined medium supplemented with a suitable lipid. High purity natural phospholipids; mono-, di-, and triglycerides; and free fatty acids are suitable lipids. The more complete lipids appear to serve simply as nutritionally convenient sources of fatty acids. T. patula can also be grown in the synthetic medium supplemented with cholesterol or other sterols in lieu of fatty acid containing lipids. Supplementation with either ethanolamine or choline permits suboptimal growth of the ciliate in a lipid-free synthetic medium. No other water soluble compound, of a variety that were tested, permitted growth of the ciliate in the lipid-free medium.