Ref ID : 3996
A. Leith and M.C. Kaufman; Partial Purification of a Species-Specific Lethal Factor from Amoeba indica. J.Protozool. 28(1):121-123, 1981
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The species-specific lethal factor obtained from Amoeba indica, a freeliving mononucleate amoeba, can be partially purified by differential centrifugation, gel exclusion chromatography, and elution from Cibacron blue-agarose. The factor is unstable in the partially purified form, but activity is correlated with a heat-labile protein an apparent molecular weight of 186,000. The factor causes inhibition of division and death when injected into A. proteus. Purification of the lethal factor indicates that aggregation of the factor with amoebal proteins is not a hindrance to further investigation. The natural role of the factor is discussed.