Ref ID : 3966
Helmut J. Schmidt; New Methods for Cultivating, Harvesting, and Purifying Mass Cultures of the Hypotrich Ciliate Euplotes aediculatus. J.Protozool. 29(1):132-135, 1982
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A new method is described for mass cultivation of Euplotes aediculatus, a hypotrich ciliate containing omikron-symbionts. The ciliate cultures, continuously aerated in Erlenmeyer flasks (5000 ml) with 4500 ml medium, yield densities of 2300 cells/ml which are four to five times higher than cell densities of cultures grown in unaerated Fernbach flasks. Harvesting such cultures involves the application of 25-µm mesh sieves. Cells so concentrated can be purified by using columns or special chambers in which Euplotes migrates towards the cathodes in an electric field (field strength 7 V/cm).